Fluorescence-Based Detection of the Desolvation Process of Protein Ions Generated in an Aqueous Electrospray Plume

Fluorescence-Based Detection of the Desolvation Process of Protein Ions Generated in an Aqueous Electrospray Plume

A new experimental setup to study laser-induced fluorescence from analytes at different locations in an electrospray plume has been developed. The high fluorescence collection efficiency (∼2%) of the setup, along with a sensitive charge coupled device (CCD) detector, enabled the study of low ion con...

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Veröffentlicht in:Analytical chemistry (Washington) 2021-02, Vol.93 (7), p.3635-3642
Hauptverfasser: Tiwari, Prince, Czar, Martin F, Zenobi, Renato
Format: Artikel
Sprache:eng
Schlagworte:
Analytical chemistry
Capillaries
Charge coupled devices
Chemistry
Diameters
Dyes
Electrospraying
Fluorescence
Ions
Laser induced fluorescence
Plumes
Proteins
Rhodamine
Spectra
Ubiquitin
Vapor phases
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Zusammenfassung:A new experimental setup to study laser-induced fluorescence from analytes at different locations in an electrospray plume has been developed. The high fluorescence collection efficiency (∼2%) of the setup, along with a sensitive charge coupled device (CCD) detector, enabled the study of low ion concentrations (down to ∼fM) in the plume. The use of small electrospray tip inner diameters (
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.0c05396