Preparation of cellulose microfibril (CMF) from Gelidium amansii and feasibility of CMF as a cosmetic ingredient

•Cellulose microfibril was extracted from Gelidium amansii, a red alga.•Microfibrillation was successfully achieved through sequential pretreatment steps.•Various physicochemical properties of CMF were investigated.•CMF inhibits UVB-induced COX-2 expression both in vitro and in vivo experiments. Cellulose microfibrils (CMF) were successfully isolated from the red alga, Gelidium amansii. G. amansii was processed in two stages, microwave digestion and high-speed blending to remove agar and extract microfibrils, respectively. After pretreatment at 180 °C for 10 min, G. amansii containing 40.1 % glucan was microfibrillated through homogenization. Morphological analysis by SEM and FTIR, and analysis of the degree of fibrillation with water retention, sedimentation, and CtCBD3 protein binding of G. amansii-derived CMF were investigated. Functional analysis of CMF showed suppression of cyclooxygenase-2 expression in both in vitro and in vivo experiments. Additionally, suppression was evident in the: i) epidermal thickness of mice skin; ii) presence of proinflammatory cytokines; and iii) inhibition of JNK1/2 and p38 phosphorylation in human keratinocyte HaCaT cells. Such activity demonstrates its anti-inflammatory properties. The results in this study showed the possibility of using CMF derived from a red alga as an anti-inflammation material.