Analysis of plasma HER2 copy number in cell-free DNA of breast cancer patients: a comparison with HER2 extracellular domain protein level in serum

Analysis of plasma HER2 copy number in cell-free DNA of breast cancer patients: a comparison with HER2 extracellular domain protein level in serum

Background HER2 (human epidermal growth factor receptor 2) status has been evaluated in breast cancer (BC) tissues by immunohistochemistry or in situ hybridization. We evaluated HER2 copy number (CN) assay in plasma cell-free DNA (cfDNA) from blood samples and compared it with protein measurements o...

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Veröffentlicht in:Breast cancer (Tokyo, Japan) Japan), 2021-05, Vol.28 (3), p.746-754
Hauptverfasser: Qui, Shi, Takeshita, Takashi, Sueta, Aiko, Tomiguchi, Mai, Goto-Yamaguchi, Lisa, Hidaka, Kaori, Suzu, Ikuko, Yamamoto, Yutaka, Iwase, Hirotaka
Format: Artikel
Sprache:eng
Schlagworte:
Breast cancer
Cancer
Cancer Research
Care and treatment
Chemotherapy
Comparative analysis
DNA
Epidermal growth factor
Genetic aspects
Genetic research
Immunohistochemistry
Medical research
Medicine
Medicine & Public Health
Medicine, Experimental
Metastasis
Monoclonal antibodies
Oncology
Original Article
Surgery
Surgical Oncology
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Zusammenfassung:Background HER2 (human epidermal growth factor receptor 2) status has been evaluated in breast cancer (BC) tissues by immunohistochemistry or in situ hybridization. We evaluated HER2 copy number (CN) assay in plasma cell-free DNA (cfDNA) from blood samples and compared it with protein measurements of HER2 extracellular domain (ECD) in serum. Methods Serum HER2-ECD levels were measured by chemi-luminescence immunoassay using anti-HER2 monoclonal antibodies. Analyses were performed on 120 cases of primary BC, 30 cases of metastatic BC and 34 cases treated by neoadjuvant chemotherapy (NAC). This study was approved by Medical Research Review Advancement No. 1857 for Kumamoto University. Results There was a positive correlation between HER2-CN ratios and HER2-ECD levels, in primary ( n  = 54) and metastatic ( n  = 30) HER2-positive BC ( P  = 0.003 and P  
ISSN:1340-6868
1880-4233
DOI:10.1007/s12282-020-01212-x