CORRELATION OF BLOOD BIOCHEMICAL INDICATORS WITH THE LEVEL OF KNEE JOINT DAMAGE IN THE MODEL OF THE POSTTRAUMATIC OSTEOARTHRITIS

The aim of the study was to assess the dependence of blood biochemical parameters with the degree of osteoarthritis of the knee joint and therapy. In experiments, osteoarthritis was simulated in rabbits (n=25) and after 75 days changes in the level of peroxidation products, activity of leukocyte enzymes were studied and the results were compared with the degree of knee joint injury, as well as taking into account the application of a concentrate of bone marrow aspirate, mechanically homogenized adipose tissue and platelet rich plasma. Analysis of the results confirmed the assumption of a direct relationship between the level of peroxidation products (diene conjugates, TBA-reactive products, products of oxidative modification of proteins), ceruloplasmin, and proinflammatory activity of leukocytes (by the activity of elastase and myeloperoxidase) with the level of morphological changes in the articular cartilage, capsule and meniscus of the knee joint. Based on histological analysis, it was concluded that the application of a concentrate of bone marrow aspirate and homogenized adipose tissue activated the processes of reorganization and regeneration of the synovial membrane and menisci of the joint, which correlated with the indicators of myeloperoxidase activity, the level of TBA-reactive products and ceruloplasmin. The action of platelet rich plasma was characterized by a correlation between leukocyte enzymes and lipid and protein oxidation products in blood serum. The results of the study suggest that the bone marrow aspirate concentrate has a greater therapeutic potential compaired to the platelet rich plasma and cell suspension obtained from adipose tissue.