Trends and applications in plant volatile sampling and analysis

SUMMARY Volatile organic compounds (VOCs) released by plants serve as information and defense chemicals in mutualistic and antagonistic interactions and mitigate effects of abiotic stress. Passive and dynamic sampling techniques combined with gas chromatography–mass spectrometry analysis have become routine tools to measure emissions of VOCs and determine their various functions. More recently, knowledge of the roles of plant VOCs in the aboveground environment has led to the exploration of similar functions in the soil and rhizosphere. Moreover, VOC patterns have been recognized as sensitive and time‐dependent markers of biotic and abiotic stress. This focused review addresses these developments by presenting recent progress in VOC sampling and analysis. We show advances in the use of small, inexpensive sampling devices and describe methods to monitor plant VOC emissions in the belowground environment. We further address latest trends in real‐time measurements of volatilomes in plant phenotyping and most recent developments of small portable devices and VOC sensors for non‐invasive VOC fingerprinting of plant disease. These technologies allow for innovative approaches to study plant VOC biology and application in agriculture. Significance Statement Determining the emission of volatile organic compounds released by plants represents a key methodology in understanding and monitoring chemical interactions of plants with their biotic and abiotic environment in agriculture and natural ecosystems. This review presents recent developments and state‐of‐the‐art technical advances in volatile organic compound sampling and analysis in belowground environments and as integrated technology of plant phenotyping and disease diagnostics.