Preparation of Stretchable Nanofibrous Sheets with Sacrificial Coaxial Electrospinning for Treatment of Traumatic Muscle Injury
Traumatic muscle injury with massive loss of muscle volume requires intramuscular implantation of proper scaffolds for fast and successful recovery. Although many artificial scaffolds effectively accelerate formation and maturation of myotubes, limited studies are showing the therapeutic effect of a...
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| Veröffentlicht in: | Advanced healthcare materials 2021-04, Vol.10 (8), p.e2002228-n/a |
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| creator | Pham‐Nguyen, Oanh‐Vu Son, Young Ju Kwon, Tae‐wan Kim, Junhyung Jung, Yun Chan Park, Jong Bae Kang, Byung‐Jae Yoo, Hyuk Sang |
| description | Traumatic muscle injury with massive loss of muscle volume requires intramuscular implantation of proper scaffolds for fast and successful recovery. Although many artificial scaffolds effectively accelerate formation and maturation of myotubes, limited studies are showing the therapeutic effect of artificial scaffolds in animal models with massive muscle injury. In this study, improved myotube differentiation is approved on stepwise stretched gelatin nanofibers and applied to damaged muscle recovery in an animal model. The gelatin nanofibers are fabricated by a two‐step process composed of co‐axial electrospinning of poly(ɛ‐caprolactone) and gelatin and subsequent removal of the outer shells. When stepwise stretching is applied to the myoblasts on gelatin nanofibers for five days, enhanced myotube formation and polarized elongation are observed. Animal models with volumetric loss at quadriceps femoris muscles (>50%) are transplanted with the myotubes cultivated on thin and flexible gelatin nanofiber. Treated animals more efficiently recover exercising functions of the leg when myotubes and the gelatin nanofiber are co‐implanted at the injury sites. This result suggests that mechanically stimulated myotubes on gelatin nanofiber is therapeutically feasible for the robust recovery of volumetric muscle loss.
Myogenesis is evaluated on step‐wise stretched gelatin nanofibers in order to apply skeletal muscle regeneration scaffolds. |
| doi_str_mv | 10.1002/adhm.202002228 |
| format | Article |
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Myogenesis is evaluated on step‐wise stretched gelatin nanofibers in order to apply skeletal muscle regeneration scaffolds.</description><identifier>ISSN: 2192-2640</identifier><identifier>EISSN: 2192-2659</identifier><identifier>DOI: 10.1002/adhm.202002228</identifier><identifier>PMID: 33506655</identifier><language>eng</language><publisher>Germany: Wiley Subscription Services, Inc</publisher><subject>Animal models ; Animals ; Cell Differentiation ; Cell Proliferation ; Electrospinning ; Elongation ; Gelatin ; Injuries ; mechanical stimulation ; Muscle Fibers, Skeletal ; muscle regeneration ; Muscles ; Myoblasts ; myogenesis ; Myotubes ; Nanofibers ; Polyesters ; Quadriceps muscle ; Recovery ; Scaffolds ; Tissue Engineering ; Tissue Scaffolds ; VML</subject><ispartof>Advanced healthcare materials, 2021-04, Vol.10 (8), p.e2002228-n/a</ispartof><rights>2021 Wiley‐VCH GmbH</rights><rights>2021 Wiley-VCH GmbH.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4108-939ba72be35ae8a615d58199aabcd67331a92c672e319dbb43dded47de8b65e83</citedby><cites>FETCH-LOGICAL-c4108-939ba72be35ae8a615d58199aabcd67331a92c672e319dbb43dded47de8b65e83</cites><orcidid>0000-0002-2557-0718 ; 0000-0002-4346-9154</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fadhm.202002228$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fadhm.202002228$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33506655$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pham‐Nguyen, Oanh‐Vu</creatorcontrib><creatorcontrib>Son, Young Ju</creatorcontrib><creatorcontrib>Kwon, Tae‐wan</creatorcontrib><creatorcontrib>Kim, Junhyung</creatorcontrib><creatorcontrib>Jung, Yun Chan</creatorcontrib><creatorcontrib>Park, Jong Bae</creatorcontrib><creatorcontrib>Kang, Byung‐Jae</creatorcontrib><creatorcontrib>Yoo, Hyuk Sang</creatorcontrib><title>Preparation of Stretchable Nanofibrous Sheets with Sacrificial Coaxial Electrospinning for Treatment of Traumatic Muscle Injury</title><title>Advanced healthcare materials</title><addtitle>Adv Healthc Mater</addtitle><description>Traumatic muscle injury with massive loss of muscle volume requires intramuscular implantation of proper scaffolds for fast and successful recovery. Although many artificial scaffolds effectively accelerate formation and maturation of myotubes, limited studies are showing the therapeutic effect of artificial scaffolds in animal models with massive muscle injury. In this study, improved myotube differentiation is approved on stepwise stretched gelatin nanofibers and applied to damaged muscle recovery in an animal model. The gelatin nanofibers are fabricated by a two‐step process composed of co‐axial electrospinning of poly(ɛ‐caprolactone) and gelatin and subsequent removal of the outer shells. When stepwise stretching is applied to the myoblasts on gelatin nanofibers for five days, enhanced myotube formation and polarized elongation are observed. Animal models with volumetric loss at quadriceps femoris muscles (>50%) are transplanted with the myotubes cultivated on thin and flexible gelatin nanofiber. Treated animals more efficiently recover exercising functions of the leg when myotubes and the gelatin nanofiber are co‐implanted at the injury sites. This result suggests that mechanically stimulated myotubes on gelatin nanofiber is therapeutically feasible for the robust recovery of volumetric muscle loss.
Myogenesis is evaluated on step‐wise stretched gelatin nanofibers in order to apply skeletal muscle regeneration scaffolds.</description><subject>Animal models</subject><subject>Animals</subject><subject>Cell Differentiation</subject><subject>Cell Proliferation</subject><subject>Electrospinning</subject><subject>Elongation</subject><subject>Gelatin</subject><subject>Injuries</subject><subject>mechanical stimulation</subject><subject>Muscle Fibers, Skeletal</subject><subject>muscle regeneration</subject><subject>Muscles</subject><subject>Myoblasts</subject><subject>myogenesis</subject><subject>Myotubes</subject><subject>Nanofibers</subject><subject>Polyesters</subject><subject>Quadriceps muscle</subject><subject>Recovery</subject><subject>Scaffolds</subject><subject>Tissue Engineering</subject><subject>Tissue Scaffolds</subject><subject>VML</subject><issn>2192-2640</issn><issn>2192-2659</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctvEzEQhy0EolXplSOyxIVLgh9rZ32sQqGV-pISzquxPUsc7SPYuyo58a_Xq7RB4tK5zBw-f-PRj5CPnM05Y-Ir-E07F0zkWYjyDTkV3IiZ0Mq8Pc4FOyHnKW1ZLq24Lvl7ciKlYlordUr-PkTcQYQh9B3ta7oaIg5uA7ZBegddXwcb-zHR1QZxSPQxDBu6AhdDHVyAhi57-DP1ywbdEPu0C10Xul-07iNdR4ShxW6YxOsIY5vXOHo7Jpft1912jPsP5F0NTcLz535Gfn6_XC-vZjf3P66XFzczV3BWzow0FhbColSAJWiuvCq5MQDWeb2QkoMRTi8ESm68tYX0Hn2x8FharbCUZ-TLwbuL_e8R01C1ITlsGugw31eJohRaS850Rj__h277MXb5d5VQXClhmJiE8wPl8tUpYl3tYmgh7ivOqimdakqnOqaTH3x61o62RX_EX7LIgDkAj6HB_Su66uLb1e0_-RM2oJ02</recordid><startdate>20210401</startdate><enddate>20210401</enddate><creator>Pham‐Nguyen, Oanh‐Vu</creator><creator>Son, Young Ju</creator><creator>Kwon, Tae‐wan</creator><creator>Kim, Junhyung</creator><creator>Jung, Yun Chan</creator><creator>Park, Jong Bae</creator><creator>Kang, Byung‐Jae</creator><creator>Yoo, Hyuk Sang</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QP</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T5</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7TO</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-2557-0718</orcidid><orcidid>https://orcid.org/0000-0002-4346-9154</orcidid></search><sort><creationdate>20210401</creationdate><title>Preparation of Stretchable Nanofibrous Sheets with Sacrificial Coaxial Electrospinning for Treatment of Traumatic Muscle Injury</title><author>Pham‐Nguyen, Oanh‐Vu ; 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Although many artificial scaffolds effectively accelerate formation and maturation of myotubes, limited studies are showing the therapeutic effect of artificial scaffolds in animal models with massive muscle injury. In this study, improved myotube differentiation is approved on stepwise stretched gelatin nanofibers and applied to damaged muscle recovery in an animal model. The gelatin nanofibers are fabricated by a two‐step process composed of co‐axial electrospinning of poly(ɛ‐caprolactone) and gelatin and subsequent removal of the outer shells. When stepwise stretching is applied to the myoblasts on gelatin nanofibers for five days, enhanced myotube formation and polarized elongation are observed. Animal models with volumetric loss at quadriceps femoris muscles (>50%) are transplanted with the myotubes cultivated on thin and flexible gelatin nanofiber. Treated animals more efficiently recover exercising functions of the leg when myotubes and the gelatin nanofiber are co‐implanted at the injury sites. This result suggests that mechanically stimulated myotubes on gelatin nanofiber is therapeutically feasible for the robust recovery of volumetric muscle loss.
Myogenesis is evaluated on step‐wise stretched gelatin nanofibers in order to apply skeletal muscle regeneration scaffolds.</abstract><cop>Germany</cop><pub>Wiley Subscription Services, Inc</pub><pmid>33506655</pmid><doi>10.1002/adhm.202002228</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-2557-0718</orcidid><orcidid>https://orcid.org/0000-0002-4346-9154</orcidid></addata></record> |
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| ispartof | Advanced healthcare materials, 2021-04, Vol.10 (8), p.e2002228-n/a |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animal models Animals Cell Differentiation Cell Proliferation Electrospinning Elongation Gelatin Injuries mechanical stimulation Muscle Fibers, Skeletal muscle regeneration Muscles Myoblasts myogenesis Myotubes Nanofibers Polyesters Quadriceps muscle Recovery Scaffolds Tissue Engineering Tissue Scaffolds VML |
| title | Preparation of Stretchable Nanofibrous Sheets with Sacrificial Coaxial Electrospinning for Treatment of Traumatic Muscle Injury |
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