Substituted meso -vinyl-BODIPY as thiol-selective fluorogenic probes for sensing unfolded proteins in the endoplasmic reticulum

Substituted meso -vinyl-BODIPY as thiol-selective fluorogenic probes for sensing unfolded proteins in the endoplasmic reticulum

A new type of thiol probes based on the meso-vinyl-BODIPY (VB) scaffold were developed. The monochloro-substituted VB1Cl exhibited the largest fluorescence enhancement (>200-fold) as well as high selectivity upon biological thiol sensing. VB1Cl was successfully applied for reporting the protein u...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Chemical communications (Cambridge, England) England), 2021-02, Vol.57 (14), p.1818-1821
Hauptverfasser: Mu, Huiying, Miki, Koji, Kubo, Takuya, Otsuka, Koji, Ohe, Kouichi
Format: Artikel
Sprache:eng
Schlagworte:
Crystallography
Endoplasmic reticulum
Fluorescence
NMR
Nuclear magnetic resonance
Proteins
Selectivity
Substitutes
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:A new type of thiol probes based on the meso-vinyl-BODIPY (VB) scaffold were developed. The monochloro-substituted VB1Cl exhibited the largest fluorescence enhancement (>200-fold) as well as high selectivity upon biological thiol sensing. VB1Cl was successfully applied for reporting the protein unfolding process under ER stress in living cells.
ISSN:1359-7345
1364-548X
DOI:10.1039/d0cc08160d