Nocardioides cynanchi sp. nov., isolated from soil of rhizosphere of Cynanchum wilfordii

A novel actinobacterial strain, SB3-45 , was isolated from soil of rhizosphere, Jaecheon-si, Chungcheongbuk-do, Republic of Korea. Strain SB3-45 , was Gram-stain-positive, aerobic and coccoid to short rod-shaped bacterium. Growth occurred at 4-37 °C (optimum 28 °C), pH 5-8 (optimum pH 7) and 0-2.5 % NaCl (optimum 0%). Phylogenetic analysis based on 16S rRNA gene sequence showed that strain SB3-45 belonged to the genus and was closely related to OS-21 (96.2%) and Gsoil 616 (95.9%). ll-DAP as the diamino acid in the peptidoglycan and the menaquinone MK-8(H ) as the predominant isoprenoid quinone were detected. The polar lipids of strain SB3-45 were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and unidentified phospholipid. The major cellular fatty acids (>5%) of strain SB3-45 were iso-C , C ω9c and C . Based on phylogenetic, physiological and chemotaxonomic characteristics, strain SB3-45 represents a novel species of the genus for which the name sp.nov. is proposed. The type strain is SB3-45 (=KCTC 49133 =NBRC 114107 ).