Human group A rotavirus P[25] VP8 specifically binds to A-type histo-blood group antigen
Rotavirus (RV) is a common cause of acute gastroenteritis in young children. While P[8] and P[4] are the most prevalent RV genotypes in humans, other genotypes are also reported in human infections occasionally, including human P[25]. The glycan binding and structural characteristics of human P[25]...
Gespeichert in:
Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 2021-03, Vol.555, p.56-63 |
---|---|
Hauptverfasser: | , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | Rotavirus (RV) is a common cause of acute gastroenteritis in young children. While P[8] and P[4] are the most prevalent RV genotypes in humans, other genotypes are also reported in human infections occasionally, including human P[25]. The glycan binding and structural characteristics of human P[25] were explored in our study. Human P[25] VP8* recognized type A histo-blood group antigen (HBGA) in the glycan microarray/oligosaccharide binding assay and could specifically hemagglutinate type A blood cells. Moreover, the P[25] VP8* structure was determined at 2.6 Å, revealing a similar conformation and a conserved putative glycan binding site as that of P[14] VP8*. This study provided further knowledge of the glycan binding and structural features of P[25] RV VP8*, promoting our understanding of the infection, prevalence, and host range of the P[III] RVs.
•Determine the crystal structure of human P[25] RV VP8* and uncover the VP8* structural characteristics.•Confirm that human P[25] VP8* specifically interacted with A-type histo-blood group antigen.•P[25] VP8* was most close to P[14] VP8* and possessed basically the same residues and conformation as that in P[14] VP8*.•A-type histo-blood group antigen may play an important role in the RV prevalence and transmission. |
---|---|
ISSN: | 0042-6822 1096-0341 |
DOI: | 10.1016/j.virol.2020.12.016 |