Age Peculiarities of Respiratory Activity and Membrane Microviscosity of Mitochondria from Rat Cardiomyocytes
We studied respiratory activity and microviscosity of mitochondrial membranes from the left ventricular myocardium of 2-month-old ( n =8) and 15-month-old ( n =8) rats. The respiratory control (RC) during substrate phosphorylation was calculated as the ratio of oxygen absorption rates in the presenc...
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| Veröffentlicht in: | Bulletin of experimental biology and medicine 2021, Vol.170 (3), p.368-370 |
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| creator | Rebrova, T. Yu Korepanov, V. A. Afanasiev, S. A. |
| description | We studied respiratory activity and microviscosity of mitochondrial membranes from the left ventricular myocardium of 2-month-old (
n
=8) and 15-month-old (
n
=8) rats. The respiratory control (RC) during substrate phosphorylation was calculated as the ratio of oxygen absorption rates in the presence of ADP and after its utilization. In 2-month-old rats, RC was 4.66 (4.56; 4.71); in 15-month-old animals, it was significantly (
р |
| doi_str_mv | 10.1007/s10517-021-05069-8 |
| format | Article |
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n
=8) and 15-month-old (
n
=8) rats. The respiratory control (RC) during substrate phosphorylation was calculated as the ratio of oxygen absorption rates in the presence of ADP and after its utilization. In 2-month-old rats, RC was 4.66 (4.56; 4.71); in 15-month-old animals, it was significantly (
р
<0.05) lower: 3.57 (3.50; 3.62). Pyrene probe eximerization indices for regions of protein-lipid and lipid-lipid interactions in mitochondria of 2-month-old animals were significantly lower than in the group of 15-month-old rats, which indicated reduced microviscosity of the lipid environment of proteins. Thus, the decrease in RC of mitochondria from the left ventricle of 15-month-old animals and the increase in the microviscosity coefficients of their membranes indicate age-related changes in the structural and functional activity of mitochondria.</description><identifier>ISSN: 0007-4888</identifier><identifier>EISSN: 1573-8221</identifier><identifier>DOI: 10.1007/s10517-021-05069-8</identifier><identifier>PMID: 33452977</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Age ; Biogerontology ; Biomedical and Life Sciences ; Biomedicine ; Cardiomyocytes ; Cell Biology ; Heart ; Internal Medicine ; Laboratory Medicine ; Lipids ; Mitochondria ; Myocardium ; Pathology ; Phosphorylation ; Proteins ; Pyrene ; Respiration ; Structure-function relationships ; Ventricle</subject><ispartof>Bulletin of experimental biology and medicine, 2021, Vol.170 (3), p.368-370</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2021</rights><rights>COPYRIGHT 2021 Springer</rights><rights>Springer Science+Business Media, LLC, part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c473t-a4badc5b0e0df411fda626d549fe4c3473da020da5d9338c2f38b19e3f1479e13</citedby><cites>FETCH-LOGICAL-c473t-a4badc5b0e0df411fda626d549fe4c3473da020da5d9338c2f38b19e3f1479e13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10517-021-05069-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10517-021-05069-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33452977$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rebrova, T. Yu</creatorcontrib><creatorcontrib>Korepanov, V. A.</creatorcontrib><creatorcontrib>Afanasiev, S. A.</creatorcontrib><title>Age Peculiarities of Respiratory Activity and Membrane Microviscosity of Mitochondria from Rat Cardiomyocytes</title><title>Bulletin of experimental biology and medicine</title><addtitle>Bull Exp Biol Med</addtitle><addtitle>Bull Exp Biol Med</addtitle><description>We studied respiratory activity and microviscosity of mitochondrial membranes from the left ventricular myocardium of 2-month-old (
n
=8) and 15-month-old (
n
=8) rats. The respiratory control (RC) during substrate phosphorylation was calculated as the ratio of oxygen absorption rates in the presence of ADP and after its utilization. In 2-month-old rats, RC was 4.66 (4.56; 4.71); in 15-month-old animals, it was significantly (
р
<0.05) lower: 3.57 (3.50; 3.62). Pyrene probe eximerization indices for regions of protein-lipid and lipid-lipid interactions in mitochondria of 2-month-old animals were significantly lower than in the group of 15-month-old rats, which indicated reduced microviscosity of the lipid environment of proteins. Thus, the decrease in RC of mitochondria from the left ventricle of 15-month-old animals and the increase in the microviscosity coefficients of their membranes indicate age-related changes in the structural and functional activity of mitochondria.</description><subject>Age</subject><subject>Biogerontology</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cardiomyocytes</subject><subject>Cell Biology</subject><subject>Heart</subject><subject>Internal Medicine</subject><subject>Laboratory Medicine</subject><subject>Lipids</subject><subject>Mitochondria</subject><subject>Myocardium</subject><subject>Pathology</subject><subject>Phosphorylation</subject><subject>Proteins</subject><subject>Pyrene</subject><subject>Respiration</subject><subject>Structure-function relationships</subject><subject>Ventricle</subject><issn>0007-4888</issn><issn>1573-8221</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kl1rFDEUhgdR7Fr9A15IQBBvpuZzJnO5LPUDuihFr0MmOdlNmZmsSaYw_96sW60VkVyE5Dzv-UjeqnpJ8AXBuH2XCBakrTElNRa46Wr5qFoR0bJaUkoeVytcqJpLKc-qZyndHI-4IU-rM8a4oF3brqpxvQP0Bcw8eB199pBQcOga0sFHnUNc0Npkf-vzgvRk0RbGPuoJ0NabGG59MiEdY0Wz9TmYfZhs9Bq5GEZ0rTPa6Gh9GJdglgzpefXE6SHBi7v9vPr2_vLr5mN99fnDp836qja8ZbnWvNfWiB4Dto4T4qxuaGMF7xxwwwpjNabYamE7xqShjsmedMAc4W0HhJ1Xb095DzF8nyFlNZZWYRhK62FOivJWik4I3Bb09V_oTZjjVLorlMSlZkfpPbXTAyg_uZCjNsekat0IIgillBfq4h9UWRZGb8IEzpf7B4I3fwj2oIe8T2GYsw9TegjSE1hePaUITh2iH3VcFMHqaAZ1MoMqZlA_zaBkEb26G23uR7C_Jb9-vwDsBKQSmnYQ72f_T9ofIGa-Eg</recordid><startdate>2021</startdate><enddate>2021</enddate><creator>Rebrova, T. Yu</creator><creator>Korepanov, V. A.</creator><creator>Afanasiev, S. 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Yu ; Korepanov, V. A. ; Afanasiev, S. A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c473t-a4badc5b0e0df411fda626d549fe4c3473da020da5d9338c2f38b19e3f1479e13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Age</topic><topic>Biogerontology</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cardiomyocytes</topic><topic>Cell Biology</topic><topic>Heart</topic><topic>Internal Medicine</topic><topic>Laboratory Medicine</topic><topic>Lipids</topic><topic>Mitochondria</topic><topic>Myocardium</topic><topic>Pathology</topic><topic>Phosphorylation</topic><topic>Proteins</topic><topic>Pyrene</topic><topic>Respiration</topic><topic>Structure-function relationships</topic><topic>Ventricle</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rebrova, T. Yu</creatorcontrib><creatorcontrib>Korepanov, V. 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Yu</au><au>Korepanov, V. A.</au><au>Afanasiev, S. A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Age Peculiarities of Respiratory Activity and Membrane Microviscosity of Mitochondria from Rat Cardiomyocytes</atitle><jtitle>Bulletin of experimental biology and medicine</jtitle><stitle>Bull Exp Biol Med</stitle><addtitle>Bull Exp Biol Med</addtitle><date>2021</date><risdate>2021</risdate><volume>170</volume><issue>3</issue><spage>368</spage><epage>370</epage><pages>368-370</pages><issn>0007-4888</issn><eissn>1573-8221</eissn><abstract>We studied respiratory activity and microviscosity of mitochondrial membranes from the left ventricular myocardium of 2-month-old (
n
=8) and 15-month-old (
n
=8) rats. The respiratory control (RC) during substrate phosphorylation was calculated as the ratio of oxygen absorption rates in the presence of ADP and after its utilization. In 2-month-old rats, RC was 4.66 (4.56; 4.71); in 15-month-old animals, it was significantly (
р
<0.05) lower: 3.57 (3.50; 3.62). Pyrene probe eximerization indices for regions of protein-lipid and lipid-lipid interactions in mitochondria of 2-month-old animals were significantly lower than in the group of 15-month-old rats, which indicated reduced microviscosity of the lipid environment of proteins. Thus, the decrease in RC of mitochondria from the left ventricle of 15-month-old animals and the increase in the microviscosity coefficients of their membranes indicate age-related changes in the structural and functional activity of mitochondria.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>33452977</pmid><doi>10.1007/s10517-021-05069-8</doi><tpages>3</tpages></addata></record> |
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| subjects | Age Biogerontology Biomedical and Life Sciences Biomedicine Cardiomyocytes Cell Biology Heart Internal Medicine Laboratory Medicine Lipids Mitochondria Myocardium Pathology Phosphorylation Proteins Pyrene Respiration Structure-function relationships Ventricle |
| title | Age Peculiarities of Respiratory Activity and Membrane Microviscosity of Mitochondria from Rat Cardiomyocytes |
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