New Class of Chitosanase from Bacillus amyloliquefaciens for the Generation of Chitooligosaccharides
Chitooligosaccharides (COS) generated from either chitin (chitin oligosaccharides) or chitosan (chitosan oligosaccharides) have a wide range of applications in agriculture, medicine, and other fields. Here, we report the characterization of a chitosanase from ( Csn) and the importance of a tryptopha...
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Veröffentlicht in: | Journal of agricultural and food chemistry 2021-01, Vol.69 (1), p.78-87 |
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Sprache: | eng |
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Zusammenfassung: | Chitooligosaccharides (COS) generated from either chitin (chitin oligosaccharides) or chitosan (chitosan oligosaccharides) have a wide range of applications in agriculture, medicine, and other fields. Here, we report the characterization of a chitosanase from
(
Csn) and the importance of a tryptophan (Trp), W204, for
Csn activity.
Csn hydrolyzed the chitosan polymer by an endo mode. It also hydrolyzed chitin oligosaccharides and interestingly exhibited transglycosylation activity on chitotetraose and chitopentaose. Mutation of W204, a nonconserved amino acid in chitosanases, to W204A abolished the hydrolytic activity of
Csn, with a change in the structure that resulted in a decreased affinity for the substrate and impaired the catalytic ability. Phylogenetic analysis revealed that
Csn could belong to a new class of chitosanases that showed unique properties like transglycosylation, cleavage of chitin oligosaccharides, and the presence of W204 residues, which is important for activity. Chitosanases belonging to the
Csn class showed a high potential to generate COS from chitinous substrates. |
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ISSN: | 0021-8561 1520-5118 |
DOI: | 10.1021/acs.jafc.0c05078 |