Vibrio marinisediminis sp. nov., Isolated from Marine Sediment

A novel Gram-stain-negative, facultative anaerobic, motile bacterium, designated as 404 T , was isolated from a marine sediment sample in the Bohai Gulf, China. Growth was observed at 10–35 °C (optimum, 20–25 °C) and in the presence of 1.0–6.0% (w/v) NaCl (optimum, 1.0–3.0%). Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain 404 T belonged to the genus Vibrio , showing the highest sequence similarity to Vibrio renipiscarius KCTC 42287 T (97.6%). The draft genome is 4.5 Mb in length, containing 4278 protein-coding genes, 60 tRNA genes and 9 rRNA genes, and DNA G+C content is 44.1 mol%. Strain 404 T contains phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine, and phospholipid as the main polar lipids, and the predominant quinone is ubiquinone Q-8. The major cellular fatty acids (>8.0%) are C 16 : 1 ω6 c and/or C 16 : 1 ω7 c , C 16 : 0 , C 18 : 1 ω6 c and/or C 18 : 1 ω7 c . Strain 404 T shows some typical characteristics among the members of genus Vibrio , while it can be clearly distinguished from the closely related type strains through genome analysis (average nucleotide identity and digital DNA–DNA hybridization values), fatty acid composition and a series of physiological and biochemical characteristics. On the basis of the polyphasic analysis, strain 404 T is considered to represent a novel species of the genus Vibrio , for which the name Vibrio marinisediminis sp. nov., is proposed. The type strain is 404 T (= MCCC 1H00367 T = KCTC 62958 T ).