Colchicine treatment early after infarction attenuates myocardial inflammatory response demonstrated by 14C-methionine imaging and subsequent ventricular remodeling by quantitative gated SPECT

Objective Colchicine has been used as an anti-inflammatory agent and may be cardioprotective after acute myocardial infarction (AMI). We investigated how colchicine administration after AMI affects the myocardial inflammatory response using 14 C-methionine and subsequent ventricular remodeling using...

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Veröffentlicht in:Annals of nuclear medicine 2021-02, Vol.35 (2), p.253-259
Hauptverfasser: Mori, Hiroshi, Taki, Junichi, Wakabayashi, Hiroshi, Hiromasa, Tomo, Inaki, Anri, Ogawa, Kazuma, Shiba, Kazuhiro, Kinuya, Seigo
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Sprache:eng
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Zusammenfassung:Objective Colchicine has been used as an anti-inflammatory agent and may be cardioprotective after acute myocardial infarction (AMI). We investigated how colchicine administration after AMI affects the myocardial inflammatory response using 14 C-methionine and subsequent ventricular remodeling using single-photon emission computed tomography (SPECT) in a rat model of AMI. Methods The left coronary artery (LCA) was occluded for 30 min followed by reperfusion. 14 C-methionine was injected at 20 min before sacrifice. The LCA was re-occluded at 1 min before sacrifice and 99m Tc-methoxyisobutylisonitrile ( 99m Tc-MIBI) was injected. Colchicine was administered intraperitoneally from day 1 to the day before 14 C-methionine injection. Dual-tracer autoradiography of the left ventricular short-axis slices was performed. The methionine uptake ratio in an ischemic area was calculated. 99m Tc-MIBI gated SPECT assessed end-diastolic volume (EDV), end-systolic volume (ESV) and left ventricular ejection fraction (LVEF). On Cluster of Differentiation 68 with 4′,6-diamidino-2-phenylindole (CD68/DAPI) staining the positive myocardial cell percentage in an ischemic area was calculated. Results In control rats, 14 C-methionine uptake ratios on day 3 and 7 were 1.87 ± 0.15 and 1.39 ± 0.12, respectively. With colchicine, the uptake was reduced on days 3 (1.56 ± 0.26, p  = 0.042) and 7 (1.23 ± 0.10, p  = 0.030). Colchicine treated rats showed smaller EDV, ESV, and higher LVEF compared with control rats. At 8 weeks, those in control rats were 864 ± 115 μL, 620 ± 100 μL, 28.4 ± 2.5%, and in colchicine rats 665 ± 75 μL, 390 ± 97 μL, 42.2 ± 8.5% ( p  = 0.012, 0.0061, 0.0083), respectively. In control rats, CD68/DAPI positive myocardial cell percentages on days 3 and 7 were 38.4 ± 1.9% and 24.0 ± 2.4%, respectively. With colchicine, the percentages were reduced significantly on both days 3 (31.5 ± 2.0%, p  
ISSN:0914-7187
1864-6433
DOI:10.1007/s12149-020-01559-3