Antioxidant effects of ankaferd blood stopper doped polyvinyl pyrolidon in an experimental model created in insect

This research evaluated Ankaferd Blood Stopper (ABS)-doped Polyvinylpyrrolidone (PVP) nanofiber layers which were produced with the electrospinning method for their potential for co-use in response to oxidative stress. As a result of the use of such a preparation (ABS doped PVP) in long-term treatments, the response to oxidative stress was compared to biochemical parameters, and its effect on sex was also aimed to be determined. For this purpose, Drosophila melanogaster foods were coated with 10% PVP, ABS (2 ml) and PVP-ABS. In total, 300 flies were randomized into 6 groups, each consisting of 25 female and 25 male insects, and the insects were fed with the determined coated mediums. The effects of foods on adult flies were tested for biochemical changes (Malondialdehyde-MDA and Total oxidation status-TOS, Glutathione-S-Transferase-GST, Catalase-CAT and Superoxide dismutase-SOD activities, Total antioxidant capacity-TAS) at the end of ten days. It was determined that the separate use of the two substances increased the amount of MDA in both sexes. It was found that the combined use of PVP-ABS had a positive effect similar to the control by increasing the antioxidant enzymes (SOD, CAT, GST). Feeding with ABS-doped PVP in the male insects reduced TOS (2.00 ± 0.01 μmol H2O2Eq/L), but the female insects were found to have higher OSI (40.00 ± 0.01 μmol H2O2Eq/L). As a result, PVP-ABS may be used together as an antioxidant, but more detailed studies are needed for their safe use on both sexes. [Display omitted] •D. melanogaster was exposed to Ankaferd Blood Stopper (ABS)-doped Polyvinylpyrrolidone (PVP) nanofiber.•The combined use of PVP-ABS increased antioxidant enzymes and ameliorated H2O2-mediated oxidative stress in flies.•ABS-PVP was not effective enough against H2O2-induced oxidative stress index in female flies.•The negative effects of separate use of nanofibers and drug with oxidative model may be reduced by combined use in flies.