Sperm head morphometry and chromatin condensation are in constant change at seminiferous tubules, epididymis, and ductus deferens in bulls

Sperm head morphometry and chromatin condensation are in constant change at seminiferous tubules, epididymis, and ductus deferens in bulls

The aim of this study was to evaluate the sperm head morphometry and chromatin condensation at different regions of the reproductive tract in bulls. Sperm smears of seminiferous tubules (ST), epididymis head (EH), body (EB), and tail (ET), and ductus deferens (DD) were stained with toluidine blue. A...

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Veröffentlicht in:Theriogenology 2021-02, Vol.161, p.200-209
Hauptverfasser: Martins, Muller Carrara, Gonçalves, Lucas Melo, Nonato, Amanda, Nassif Travençolo, Bruno Augusto, Alves, Benner Geraldo, Beletti, Marcelo Emílio
Format: Artikel
Sprache:eng
Schlagworte:
Chromatin condensation
Epididymis
Sperm morphometry
Toluidine blue
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Zusammenfassung:The aim of this study was to evaluate the sperm head morphometry and chromatin condensation at different regions of the reproductive tract in bulls. Sperm smears of seminiferous tubules (ST), epididymis head (EH), body (EB), and tail (ET), and ductus deferens (DD) were stained with toluidine blue. Afterwards, the sperm head morphometry and chromatin alteration types were evaluated by a computational image analysis. Overall, spermatozoa of ST had lower (P 
ISSN:0093-691X
1879-3231
DOI:10.1016/j.theriogenology.2020.12.004