One-pot preparation of a multi-functional enzymatically generated gelatin hydrogel with controllable antibacterial and hemorheological properties

The creation of multi-functional bio-hydrogels with tunable properties that meet in vivo demands is significant but remains challenging. Inspired by host–guest chemistry, a novel multi-functional gelatin-based bio-hydrogel with tunable antibacterial and hemorheological properties (TAH-GEL) is synthesized via an in situ one-pot strategy. TAH-GEL not only exhibits excellent mechanical properties but also shows promising self-healing and bio-compatibility features. For the first time, this biomaterial presents controllable antibacterial and hemorheological properties by controlling the TAH-GEL polypseudorotaxane motif. The resulting bio-hydrogel is easy to prepare and delivers superior performance, making it a powerful tool for bio-applications, such as hemostatic materials. [Display omitted] •A novel multi-functional gelatin-based bio-hydrogel was fabricated via an in situ one-pot strategy.•The bio-hydrogel can mimick the extracellular matrix component of human tissues and organs.•The tensile strength of the bio-hydrogel increased by 2378% compared with the blank gelatin.•ε-PLL and cucurbit[7] uril were used to provide the bio-hydrogels tunable antibacterial and hemorheological properties.•Coagulation and anti-coagulation properties of the bio-hydrogel can be tunable to meet different physiological needs.