Environmental Liquid Cell Technique for Improved Electron Microscopic Imaging of Soft Matter in Solution

Liquid-phase transmission electron microscopy is a technique for simultaneous imaging of the structure and dynamics of specimens in a liquid environment. The conventional sample geometry consists of a liquid layer tightly sandwiched between two Si3N4 windows with a nominal spacing on the order of 0....

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Veröffentlicht in:Microscopy and microanalysis 2021-02, Vol.27 (1), p.44-53
Hauptverfasser: Azim, Sana, Bultema, Lindsey A., de Kock, Michiel B., Osorio-Blanco, Ernesto Rafael, Calderón, Marcelo, Gonschior, Josef, Leimkohl, Jan-Philipp, Tellkamp, Friedjof, Bücker, Robert, Schulz, Eike C., Keskin, Sercan, de Jonge, Niels, Kassier, Günther H., Miller, R.J. Dwayne
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Sprache:eng
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Zusammenfassung:Liquid-phase transmission electron microscopy is a technique for simultaneous imaging of the structure and dynamics of specimens in a liquid environment. The conventional sample geometry consists of a liquid layer tightly sandwiched between two Si3N4 windows with a nominal spacing on the order of 0.5 μm. We describe a variation of the conventional approach, wherein the Si3N4 windows are separated by a 10-μm-thick spacer, thus providing room for gas flow inside the liquid specimen enclosure. Adjusting the pressure and flow speed of humid air inside this environmental liquid cell (ELC) creates a stable liquid layer of controllable thickness on the bottom window, thus facilitating high-resolution observations of low mass-thickness contrast objects at low electron doses. We demonstrate controllable liquid thicknesses in the range 160 ± 34 to 340 ± 71 nm resulting in corresponding edge resolutions of 0.8 ± 0.06 to 1.7 ± 0.8 nm as measured for immersed gold nanoparticles. Liquid layer thickness 40 ± 8 nm allowed imaging of low-contrast polystyrene particles. Hydration effects in the ELC have been studied using poly-N-isopropylacrylamide nanogels with a silica core. Therefore, ELC can be a suitable tool for in situ investigations of liquid specimens.
ISSN:1431-9276
1435-8115
DOI:10.1017/S1431927620024654