Hollow MnFeO oxide derived from MOF@MOF with multiple enzyme-like activities for multifunction colorimetric assay of biomolecules and Hg2

In this work, a new type of hollow MnFeO oxide derived obtained from the metal-organic framework (MOF)@MOF was designed and proposed, which has intrinsic activity of mimicking enzymes of oxidase and peroxidase by adjustment pH values. Based on the colorimetric reaction and the hindrance of the oxidase-like activity in the presence of L-cysteine (Cys), as well as the recovery of oxidase-like activity due to the specific complexation of Cys and mercury (II) ions (Hg2+), a new type of colorimetric transmission platform for Cys and Hg2+ detection with wide linear ranges of 1–25 μM for Cys and 0.1–15 μM for Hg2+ has been developed. Besides, a better colorimetric sensing platform for detecting H2O2 was established with linear ranges of 1–60 μM and 60–300 μM based on generating hydroxyl radicals (·OH). Furthermore, the hollow MnFeO oxide has high stability, excellent selectivity with good activity over a long period of time. Surprisingly, the proposed method for Cys, Hg2+ and H2O2 estimation can also be used in actual samples. These characteristics lay a foundation for further investigation about the catalytic activity of the hollow MnFeO oxide nanomaterials and make it show broad prospects in the field of biosensing and catalysis. [Display omitted] •A hollow MnFeO oxide derived from MOF@MOF is synthesized and has multi-enzyme simulation activity. •pH adjustment to avoid mutual interference between multiple enzyme activities. •Sensitive colorimetric detection of Cys, Hg2+ and H2O2 using different simulated enzyme activities of hollow MnFeO oxide. •This method is used for the detection of Cys in fetal bovine serum samples and Hg2+ in water samples.