Aptamer protective groups tolerate different reagents and reactions for regioselective modification of neomycin B

The aptameric protective group strategy for the one-step regioselective transformation of aminoglycoside antibiotics was found to be compatible with diverse reagents and reaction conditions. New derivatives of neomycin B were synthesized with regioselectivities of >99%. This result extends the scope of applicability of APGs facilitating access to novel aminoglycoside derivatives. The aptameric protective group strategy is compatible with diverse reagents and reaction conditions for the synthesis of new neomycin B derivatives.