Electrochemical DNA detection of hepatitis E virus genotype 3 using PbS quantum dot labelling

Electrochemical DNA detection of hepatitis E virus genotype 3 using PbS quantum dot labelling

The aim of this study was to develop a highly specific electrochemical DNA sensor using functionalized lead sulphide (PbS) quantum dots for hepatitis E virus genotype 3 ( HEV3) DNA target detection. Functionalized-PbS quantum dots (QDs) were used as an electrochemical label for the detection of HEV3...

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Veröffentlicht in:Analytical and bioanalytical chemistry 2021-02, Vol.413 (4), p.1027-1037
Hauptverfasser: Ngo, Duy Ba, Chaibun, Thanyarat, Yin, Lee Su, Lertanantawong, Benchaporn, Surareungchai, Werasak
Format: Artikel
Sprache:eng
Schlagworte:
Affinity labeling
Analytical Chemistry
Anodic stripping
Biochemistry
Biosensors
Characterization and Evaluation of Materials
Chemical properties
Chemistry
Chemistry and Materials Science
Deoxyribonucleic acid
DNA
Electrochemical analysis
Electrochemistry
Food Science
Gene targeting
Genetic aspects
Genotypes
Hepatitis
Hepatitis E virus
Identification and classification
Labeling
Laboratory Medicine
Lead compounds
Lead sulfides
Materials
Methods
Monitoring/Environmental Analysis
Quantum dots
Research Paper
Square waves
Sulfides
Target detection
Zeta potential
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Zusammenfassung:The aim of this study was to develop a highly specific electrochemical DNA sensor using functionalized lead sulphide (PbS) quantum dots for hepatitis E virus genotype 3 ( HEV3) DNA target detection. Functionalized-PbS quantum dots (QDs) were used as an electrochemical label for the detection of HEV3-DNA target by the technique of square wave anodic stripping voltammetry ( SWASV). The functionalized-PbS quantum dots were characterized by UV-vis, FTIR, XRD, TEM and zeta potential techniques. As-prepared, functionalized-PbS quantum dots have an average size of 4.15 ± 1.35 nm. The detection platform exhibited LOD and LOQ values of 1.23 fM and 2.11 fM, respectively. HEV3-DNA target spiked serum is also reported. Graphical abstract
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-020-03061-1