Correlation of Immunomodulatory Cytokine Expression with Histopathological Changes and Viral Antigen in a Hamster Model of Equine Herpesvirus-9 Encephalitis

A group of hamsters (n = 25) was intranasally infected with equine herpesvirus-9 (EHV-9) and mRNA transcription levels of several proinflammatory (IFN-γ, TNF-α and IL-6) and anti-inflammatory (IL-4, IL-10 and TGF-β) cytokines were investigated in brain tissue using RT-qPCR. These levels were correla...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of comparative pathology 2020-10, Vol.180, p.46-54
Hauptverfasser:	Abd-Ellatieff, Hoda, Anwar, Shehata, Abas, Osama, Abou-Rawash, Abdel-Rhman, Fukushi, Hiadeto, Yanai, Tokuma
Format:	Artikel
Sprache:	eng
Schlagworte:	cytokines equine herpesvirus-9 meningoencephalitis RT-qPCR
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

container_end_page	54
container_issue
container_start_page	46
container_title	Journal of comparative pathology
container_volume	180
creator	Abd-Ellatieff, Hoda Anwar, Shehata Abas, Osama Abou-Rawash, Abdel-Rhman Fukushi, Hiadeto Yanai, Tokuma
description	A group of hamsters (n = 25) was intranasally infected with equine herpesvirus-9 (EHV-9) and mRNA transcription levels of several proinflammatory (IFN-γ, TNF-α and IL-6) and anti-inflammatory (IL-4, IL-10 and TGF-β) cytokines were investigated in brain tissue using RT-qPCR. These levels were correlated with the severity of sequential histopathological changes and intensity of immunohistochemical labelling of virus antigen in brain. Early and progressive upregulation of all the proinflammatory and anti-inflammatory cytokines investigated (P
doi_str_mv	10.1016/j.jcpa.2020.07.007
format	Article
fullrecord	<record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2463608015</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0021997520300815</els_id><sourcerecordid>2463608015</sourcerecordid><originalsourceid>FETCH-LOGICAL-c333t-d94d69c0a1a9d127c75846c7cdec67bcfe74736a823a78c518c96fd6d23857013</originalsourceid><addsrcrecordid>eNp9kUGP0zAQhS0EEqXwBzj5yCVhbDdxInFZRYWutIgLu1fLa09al8TO2s6y_S_8WBKVM6eRnt57o5mPkI8MSgas_nwuz2bSJQcOJcgSQL4iG1YJXgjG29dkA8BZ0bayekvepXQGgKap-Ib86UKMOOjsgqehp7fjOPswBjsvWogX2l1y-OU80v3LFDGl1ffb5RM9uJTDpPMpDOHojB5od9L-iIlqb-mDi4ty47M7oqfOU00PekwZI_0eLA7rrv3TvBYfME6Ynl2cU9HSvTc4nfTgskvvyZteDwk__Jtbcv91_7M7FHc_vt12N3eFEULkwrY7W7cGNNOtZVwaWTW72khj0dTy0fQod1LUuuFCy8ZUrDFt3dvactFUEpjYkk_X3imGpxlTVqNLBodBewxzUnxXixoaWB66JfxqNTGkFLFXU3SjjhfFQK0o1FmtKNSKQoFUC4ol9OUawuWIZ4dRJeNwOdS6iCYrG9z_4n8BJLqViQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2463608015</pqid></control><display><type>article</type><title>Correlation of Immunomodulatory Cytokine Expression with Histopathological Changes and Viral Antigen in a Hamster Model of Equine Herpesvirus-9 Encephalitis</title><source>Access via ScienceDirect (Elsevier)</source><creator>Abd-Ellatieff, Hoda ; Anwar, Shehata ; Abas, Osama ; Abou-Rawash, Abdel-Rhman ; Fukushi, Hiadeto ; Yanai, Tokuma</creator><creatorcontrib>Abd-Ellatieff, Hoda ; Anwar, Shehata ; Abas, Osama ; Abou-Rawash, Abdel-Rhman ; Fukushi, Hiadeto ; Yanai, Tokuma</creatorcontrib><description>A group of hamsters (n = 25) was intranasally infected with equine herpesvirus-9 (EHV-9) and mRNA transcription levels of several proinflammatory (IFN-γ, TNF-α and IL-6) and anti-inflammatory (IL-4, IL-10 and TGF-β) cytokines were investigated in brain tissue using RT-qPCR. These levels were correlated with the severity of sequential histopathological changes and intensity of immunohistochemical labelling of virus antigen in brain. Early and progressive upregulation of all the proinflammatory and anti-inflammatory cytokines investigated (P < 0.05) was correlated with increasing severity of encephalitis and viral antigen expression from 2 days post infection (dpi) with a peak at 4–5 dpi (P <0.05).</description><identifier>ISSN: 0021-9975</identifier><identifier>EISSN: 1532-3129</identifier><identifier>DOI: 10.1016/j.jcpa.2020.07.007</identifier><language>eng</language><publisher>Elsevier Ltd</publisher><subject>cytokines ; equine herpesvirus-9 ; meningoencephalitis ; RT-qPCR</subject><ispartof>Journal of comparative pathology, 2020-10, Vol.180, p.46-54</ispartof><rights>2020 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c333t-d94d69c0a1a9d127c75846c7cdec67bcfe74736a823a78c518c96fd6d23857013</citedby><cites>FETCH-LOGICAL-c333t-d94d69c0a1a9d127c75846c7cdec67bcfe74736a823a78c518c96fd6d23857013</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jcpa.2020.07.007$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids></links><search><creatorcontrib>Abd-Ellatieff, Hoda</creatorcontrib><creatorcontrib>Anwar, Shehata</creatorcontrib><creatorcontrib>Abas, Osama</creatorcontrib><creatorcontrib>Abou-Rawash, Abdel-Rhman</creatorcontrib><creatorcontrib>Fukushi, Hiadeto</creatorcontrib><creatorcontrib>Yanai, Tokuma</creatorcontrib><title>Correlation of Immunomodulatory Cytokine Expression with Histopathological Changes and Viral Antigen in a Hamster Model of Equine Herpesvirus-9 Encephalitis</title><title>Journal of comparative pathology</title><description>A group of hamsters (n = 25) was intranasally infected with equine herpesvirus-9 (EHV-9) and mRNA transcription levels of several proinflammatory (IFN-γ, TNF-α and IL-6) and anti-inflammatory (IL-4, IL-10 and TGF-β) cytokines were investigated in brain tissue using RT-qPCR. These levels were correlated with the severity of sequential histopathological changes and intensity of immunohistochemical labelling of virus antigen in brain. Early and progressive upregulation of all the proinflammatory and anti-inflammatory cytokines investigated (P < 0.05) was correlated with increasing severity of encephalitis and viral antigen expression from 2 days post infection (dpi) with a peak at 4–5 dpi (P <0.05).</description><subject>cytokines</subject><subject>equine herpesvirus-9</subject><subject>meningoencephalitis</subject><subject>RT-qPCR</subject><issn>0021-9975</issn><issn>1532-3129</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kUGP0zAQhS0EEqXwBzj5yCVhbDdxInFZRYWutIgLu1fLa09al8TO2s6y_S_8WBKVM6eRnt57o5mPkI8MSgas_nwuz2bSJQcOJcgSQL4iG1YJXgjG29dkA8BZ0bayekvepXQGgKap-Ib86UKMOOjsgqehp7fjOPswBjsvWogX2l1y-OU80v3LFDGl1ffb5RM9uJTDpPMpDOHojB5od9L-iIlqb-mDi4ty47M7oqfOU00PekwZI_0eLA7rrv3TvBYfME6Ynl2cU9HSvTc4nfTgskvvyZteDwk__Jtbcv91_7M7FHc_vt12N3eFEULkwrY7W7cGNNOtZVwaWTW72khj0dTy0fQod1LUuuFCy8ZUrDFt3dvactFUEpjYkk_X3imGpxlTVqNLBodBewxzUnxXixoaWB66JfxqNTGkFLFXU3SjjhfFQK0o1FmtKNSKQoFUC4ol9OUawuWIZ4dRJeNwOdS6iCYrG9z_4n8BJLqViQ</recordid><startdate>202010</startdate><enddate>202010</enddate><creator>Abd-Ellatieff, Hoda</creator><creator>Anwar, Shehata</creator><creator>Abas, Osama</creator><creator>Abou-Rawash, Abdel-Rhman</creator><creator>Fukushi, Hiadeto</creator><creator>Yanai, Tokuma</creator><general>Elsevier Ltd</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202010</creationdate><title>Correlation of Immunomodulatory Cytokine Expression with Histopathological Changes and Viral Antigen in a Hamster Model of Equine Herpesvirus-9 Encephalitis</title><author>Abd-Ellatieff, Hoda ; Anwar, Shehata ; Abas, Osama ; Abou-Rawash, Abdel-Rhman ; Fukushi, Hiadeto ; Yanai, Tokuma</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c333t-d94d69c0a1a9d127c75846c7cdec67bcfe74736a823a78c518c96fd6d23857013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>cytokines</topic><topic>equine herpesvirus-9</topic><topic>meningoencephalitis</topic><topic>RT-qPCR</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Abd-Ellatieff, Hoda</creatorcontrib><creatorcontrib>Anwar, Shehata</creatorcontrib><creatorcontrib>Abas, Osama</creatorcontrib><creatorcontrib>Abou-Rawash, Abdel-Rhman</creatorcontrib><creatorcontrib>Fukushi, Hiadeto</creatorcontrib><creatorcontrib>Yanai, Tokuma</creatorcontrib><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of comparative pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abd-Ellatieff, Hoda</au><au>Anwar, Shehata</au><au>Abas, Osama</au><au>Abou-Rawash, Abdel-Rhman</au><au>Fukushi, Hiadeto</au><au>Yanai, Tokuma</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Correlation of Immunomodulatory Cytokine Expression with Histopathological Changes and Viral Antigen in a Hamster Model of Equine Herpesvirus-9 Encephalitis</atitle><jtitle>Journal of comparative pathology</jtitle><date>2020-10</date><risdate>2020</risdate><volume>180</volume><spage>46</spage><epage>54</epage><pages>46-54</pages><issn>0021-9975</issn><eissn>1532-3129</eissn><abstract>A group of hamsters (n = 25) was intranasally infected with equine herpesvirus-9 (EHV-9) and mRNA transcription levels of several proinflammatory (IFN-γ, TNF-α and IL-6) and anti-inflammatory (IL-4, IL-10 and TGF-β) cytokines were investigated in brain tissue using RT-qPCR. These levels were correlated with the severity of sequential histopathological changes and intensity of immunohistochemical labelling of virus antigen in brain. Early and progressive upregulation of all the proinflammatory and anti-inflammatory cytokines investigated (P < 0.05) was correlated with increasing severity of encephalitis and viral antigen expression from 2 days post infection (dpi) with a peak at 4–5 dpi (P <0.05).</abstract><pub>Elsevier Ltd</pub><doi>10.1016/j.jcpa.2020.07.007</doi><tpages>9</tpages></addata></record>
fulltext	fulltext
identifier	ISSN: 0021-9975
ispartof	Journal of comparative pathology, 2020-10, Vol.180, p.46-54
issn	0021-9975 1532-3129
language	eng
recordid	cdi_proquest_miscellaneous_2463608015
source	Access via ScienceDirect (Elsevier)
subjects	cytokines equine herpesvirus-9 meningoencephalitis RT-qPCR
title	Correlation of Immunomodulatory Cytokine Expression with Histopathological Changes and Viral Antigen in a Hamster Model of Equine Herpesvirus-9 Encephalitis
url	https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-28T22%3A54%3A26IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Correlation%20of%20Immunomodulatory%20Cytokine%20Expression%20with%20Histopathological%20Changes%20and%20Viral%20Antigen%20in%20a%20Hamster%20Model%20of%20Equine%20Herpesvirus-9%20Encephalitis&rft.jtitle=Journal%20of%20comparative%20pathology&rft.au=Abd-Ellatieff,%20Hoda&rft.date=2020-10&rft.volume=180&rft.spage=46&rft.epage=54&rft.pages=46-54&rft.issn=0021-9975&rft.eissn=1532-3129&rft_id=info:doi/10.1016/j.jcpa.2020.07.007&rft_dat=%3Cproquest_cross%3E2463608015%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2463608015&rft_id=info:pmid/&rft_els_id=S0021997520300815&rfr_iscdi=true