Correlation of Immunomodulatory Cytokine Expression with Histopathological Changes and Viral Antigen in a Hamster Model of Equine Herpesvirus-9 Encephalitis

Correlation of Immunomodulatory Cytokine Expression with Histopathological Changes and Viral Antigen in a Hamster Model of Equine Herpesvirus-9 Encephalitis

A group of hamsters (n = 25) was intranasally infected with equine herpesvirus-9 (EHV-9) and mRNA transcription levels of several proinflammatory (IFN-γ, TNF-α and IL-6) and anti-inflammatory (IL-4, IL-10 and TGF-β) cytokines were investigated in brain tissue using RT-qPCR. These levels were correla...

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Veröffentlicht in:Journal of comparative pathology 2020-10, Vol.180, p.46-54
Hauptverfasser: Abd-Ellatieff, Hoda, Anwar, Shehata, Abas, Osama, Abou-Rawash, Abdel-Rhman, Fukushi, Hiadeto, Yanai, Tokuma
Format: Artikel
Sprache:eng
Schlagworte:
cytokines
equine herpesvirus-9
meningoencephalitis
RT-qPCR
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Zusammenfassung:A group of hamsters (n = 25) was intranasally infected with equine herpesvirus-9 (EHV-9) and mRNA transcription levels of several proinflammatory (IFN-γ, TNF-α and IL-6) and anti-inflammatory (IL-4, IL-10 and TGF-β) cytokines were investigated in brain tissue using RT-qPCR. These levels were correlated with the severity of sequential histopathological changes and intensity of immunohistochemical labelling of virus antigen in brain. Early and progressive upregulation of all the proinflammatory and anti-inflammatory cytokines investigated (P 
ISSN:0021-9975
1532-3129
DOI:10.1016/j.jcpa.2020.07.007