Effects of phosphogypsum and medical stone on nitrogen transformation, nitrogen functional genes, and bacterial community during aerobic composting

This study explored the effects of adding phosphogypsum (PPG), medical stone (MS), and both (PPM) during composting on nitrogen transformation, nitrogen functional genes, the bacterial community, and their relationships with NH3 and N2O emissions. Adding MS and PPM reduced NH3 emissions by 25.78–68.37% and N2O emissions by 19.00–42.86%. PPG reduced NH3 emissions by 59.74% but slightly increased N2O emissions by 8.15%. MS was strongly correlated with the amoA-dominated nitrification process. PPG and PPM had strong correlations with nirS- and nirK-dominated, and nosZ-dominated denitrification processes, respectively. PPM promoted nitrification and denitrification processes more than PPG and MS. Different functional bacteria had key roles in nitrification and denitrification during different composting stages. Firmicutes probably contributed to the conversion and release of nitrogen in the thermophilic period, whereas Proteobacteria, Chloroflexi, Bacteroidetes, and other phyla might have played important roles in the cooling and maturation periods. PPM obtained the greatest reductions in NH3 and N2O release via the regulation of environmental variables, nitrogen functional genes, and the bacterial community. Overall, these results provide insights at a molecular level into the effects of PPG and MS on nitrogen transformation and NH3 and N2O emissions during composting. [Display omitted] •Adding phosphogypsum and medical stone effectively reduced NH3 and N2O emissions.•Medical stone mainly affected the amoA-dominated nitrification process.•Phosphogypsum mostly affected the nirS- and nirK- dominated denitrification process.•Additives had different effects on functional bacteria in various composting stages.•Firmicutes and Proteobacteria made major contributions to nitrogen conversion.