Ornithinimicrobium pratense sp. nov., isolated from meadow soil
A novel Gram-stain-positive, yellow, short-rod-shaped or coccoid bacterial strain, W204 , was isolated from a soil sample collected from Jiadengyu national forest park in China and characterized using a polyphasic approach. The cell-wall peptidoglycan contained ornithine as the diagnostic diamino ac...
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Veröffentlicht in: | International journal of systematic and evolutionary microbiology 2020-12, Vol.70 (12), p.6450-6457 |
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Sprache: | eng |
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Zusammenfassung: | A novel Gram-stain-positive, yellow, short-rod-shaped or coccoid bacterial strain, W204
, was isolated from a soil sample collected from Jiadengyu national forest park in China and characterized using a polyphasic approach. The cell-wall peptidoglycan contained ornithine as the diagnostic diamino acid. 16S rRNA gene sequence analysis indicated that strain W204
was closely related to
CPCC 203535
(97.4 %, similarity),
CGMCC 4.5582
(96.9 %),
GP-T3-3
(96.8 %),
JLT9
(96.7 %),
CPCC 203383
(96.6 %) and
K22-20
(96.6 %). However, the digital DNA-DNA genome hybridization value between strain W204
and the closest related strain
CPCC 203535
was 21.90 %. Complete genome analyses revealed that the size of the genome was 3.54 Mb and the genomic DNA G+C content was 70.79 mol%. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, an unidentified glycolipid, an unidentified phospholipid and an unidentified lipid. The major menaquinone was MK-8(H
). The predominant cellular fatty acids were iso-C
, anteiso-C
and C
. The phenotypic, chemotaxonomic and phylogenetic data suggested that strain W204
should be classified as representative of a novel species of the genus
, for which the name
sp. nov. is proposed. The type strain is W204
(=GDMCC 1.1391
=KCTC 49237
). |
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ISSN: | 1466-5026 1466-5034 |
DOI: | 10.1099/ijsem.0.004557 |