Ornithinimicrobium pratense sp. nov., isolated from meadow soil

A novel Gram-stain-positive, yellow, short-rod-shaped or coccoid bacterial strain, W204 , was isolated from a soil sample collected from Jiadengyu national forest park in China and characterized using a polyphasic approach. The cell-wall peptidoglycan contained ornithine as the diagnostic diamino ac...

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Veröffentlicht in:International journal of systematic and evolutionary microbiology 2020-12, Vol.70 (12), p.6450-6457
Hauptverfasser: Guo, Qiannan, Wang, Bo, Zhou, Zhengfu, Ke, Xiubin, Zhang, Lijuan, Chen, Ming, Lin, Min, Wang, Wei, Zhang, Wei, Li, Xufeng
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Sprache:eng
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Zusammenfassung:A novel Gram-stain-positive, yellow, short-rod-shaped or coccoid bacterial strain, W204 , was isolated from a soil sample collected from Jiadengyu national forest park in China and characterized using a polyphasic approach. The cell-wall peptidoglycan contained ornithine as the diagnostic diamino acid. 16S rRNA gene sequence analysis indicated that strain W204 was closely related to CPCC 203535 (97.4 %, similarity), CGMCC 4.5582 (96.9 %), GP-T3-3 (96.8 %), JLT9 (96.7 %), CPCC 203383 (96.6 %) and K22-20 (96.6 %). However, the digital DNA-DNA genome hybridization value between strain W204 and the closest related strain CPCC 203535 was 21.90 %. Complete genome analyses revealed that the size of the genome was 3.54 Mb and the genomic DNA G+C content was 70.79 mol%. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, an unidentified glycolipid, an unidentified phospholipid and an unidentified lipid. The major menaquinone was MK-8(H ). The predominant cellular fatty acids were iso-C , anteiso-C and C . The phenotypic, chemotaxonomic and phylogenetic data suggested that strain W204 should be classified as representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is W204 (=GDMCC 1.1391 =KCTC 49237 ).
ISSN:1466-5026
1466-5034
DOI:10.1099/ijsem.0.004557