Development of a fragmentation pattern of synthetic cannabinoids based on electrospray ionization mass spectrometry in positive ion mode to screen synthetic cannabinoids in illicit products

[Display omitted] •The MS fragmentation pathways of twenty-seven synthetic cannabinoids were studied.•The MS fragmentation pattern of synthetic cannabinoids was summarized.•New evidence for the rapid structure identification of synthetic cannabinoids.•Rapid screening of synthetic cannabinoids in real samples from drug cases. To explore the MS fragmentation pattern of synthetic cannabinoids by electrospray ionization mass spectrometry, twenty-seven synthetic cannabinoids were systematically investigated by liquid chromatography coupled to high-resolution quadrupole Orbitrap mass spectrometry(LC-Q-Orbitrap/MS)with positive mode of electrospray ionization. Based on tandem multistage MS and high resolution MS data, MS fragmentation pattern of synthetic cannabinoids was summarized. The cleavage of CC bonds next to the oxygen at the side chain on the C-3 position of synthetic cannabinoids was the characteristic fragmentation pathway of synthetic cannabinoids in the positive mode of electrospray ionization. When the synthetic cannabinoids with a 3-carbamoylpropyl-indole/indazole structure, NH3, CO, NH2CHO and CH2(CH3)2 were easy to lose to form different ions. While when the synthetic cannabinoids with a 3-carboxamide-indole/indazole structure, the side chain on the C-3 position was susceptible to γ-cleavage. In addition, this MS fragmentation pattern was applied to quickly screen whether electronic cigarette oil and tobacco from drug cases contain synthetic cannabinoids. This kind of compounds had strong fragmentation pattern, which provided new evidence for the rapid structure identification of synthetic cannabinoids.