Biosafety Level 2 cabinet UV‐C light exposure of sports antidoping human urine samples does not affect the stability of selected prohibited substances

The current study examined the stability of several antidoping prohibited substances analytes in urine after 15‐min exposure to UV‐C light in a Biosafety Level 2 cabinet. The urine matrices were exposed within the original antidoping bottles with the aim to destroy DNA/RNA and possible SARS CoV‐2. The analytes small molecules Phase I and Phase II metabolites and peptides, in total 444, endogenous, internal standards, and prohibited substances, pH, and specific gravity in urine were studied. The accredited analytical methods were used by Anti‐Doping Laboratory Qatar for the comparison of data of the same urine samples analyzed with and without UV‐C exposure. In the study conditions, no problems of stability were detected in the substances spiked in the urine samples exposed in the UV‐C irradiation. Biosafety Level 2 cabinet UV‐C light exposure of sports antidoping human urine samples does not affect the stability of selected prohibited substances studied in samples with and without irradiation. The UV‐C irradiation is degrading DNA/RNA and coronaviruses. The biosafety Level 2 cabinet use has been recommended by WADA.