Physicochemical characterization and self-assembly of human amniotic membrane and umbilical cord collagen: A comparative study

The diverse application of collagen has created a need to discover renewable and economical sources with prevailing/improved physico-chemical properties. To address this scenario, the present study has extracted collagen from Human Amniotic Membrane (AM) and Umbilical cord, which are treated as medi...

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Veröffentlicht in:International journal of biological macromolecules 2020-12, Vol.165 (Pt B), p.2920-2933
Hauptverfasser: Gunasekaran, Deebasuganya, Thada, Rajarajeshwari, Jeyakumar, Grace Felciya Sekar, Manimegalai, Nivethitha Panneerselvam, Shanmugam, Ganesh, Sivagnanam, Uma Tiruchirapalli
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Sprache:eng
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Zusammenfassung:The diverse application of collagen has created a need to discover renewable and economical sources with prevailing/improved physico-chemical properties. To address this scenario, the present study has extracted collagen from Human Amniotic Membrane (AM) and Umbilical cord, which are treated as medical waste and compared its physico-chemical properties. Collagen was extracted by pepsin solubilization using various salt concentrations (1 M, 2 M and 4 M). Umbilical Cord Collagen (UC) yield was 10% higher than Amniotic Membrane Collagen (AC). UC reported 58% higher sulphated glycosaminoglycan content than AC. Electrophoretic pattern of AC and UC in both disulphide bond reducing and non-reducing conditions showed bands corresponding to collagen type I, III, IV, V and XV. Collagen morphology was examined using SEM and the amino acid content was quantified by HPLC and LC-MS/MS. Triple helicity was confirmed by CD and FTIR spectra. Thermal transition temperature of AC and UC was found equivalent to animal collagen. Self-assembly, fibril morphology and spatial alignment was studied using AFM and DLS. Biocompatibility was analyzed using 3T3 fibroblast cells. In conclusion, UC with higher yield, presented with better physico-chemical, structural and biological properties than AC could serve as an efficient alternative to the existing animal collagen for diverse applications. [Display omitted] •The study utilized postpartum waste for collagen isolation.•Molecular weight of collagen types in amnion and umbilical cord was identified.•Collagens showed self-assembling ability and cytocompatibility.•Large size and overlap region of umbilical cord collagen fibril imparts stability.•Umbilical cord would be a better collagen source than amnion for clinical therapy.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2020.10.107