In vivo embryo production and recovery in lacaune ewes after imposing a superovulation treatment regimen is related to pFSH dose

•Estrous response and corpus luteum count were lower in ewes treated with 100 mg pFSH.•200 mg pFSH resulted in greater superovulatory response and embryo yield•100 mg pFSH was insufficient to promote superovulation in lactating Lacaune ewes•good ova/embryo recovery rate was observed only with 200 mg pFSH treatment. This study was conducted to assess effects of different doses of pFSH on follicular recruitment, superovulatory response, ova/embryo recovery, and embryo yield in lactating ewes. Ewes (n = 24) had a superovulation treatment regimen imposed. All ewes were implanted with a progesterone intravaginal device for 9 d, and administered either 100 (G-100) or 200 (G-200) mg pFSH, proportioned into six doses administered at 12-h intervals, starting 60 h before device removal. At 7 days subsequent to progesterone device removal, there were non-surgical embryo recoveries (NSER) from ewes having three or more corpora lutea. At the time of the first pFSH injection, number of antral follicles were similar (P