A shift in the pool of retained microphytobenthos nitrogen under enhanced nutrient availability

Sediment microbial communities are an important sink for both organic and inorganic nitrogen (N), with microphytobenthos (MPB) biomass making the largest contribution to short-term N-assimilation and retention. Coastal waters are increasingly subject to anthropogenic nutrient enrichment, but the effect of nutrient enrichment on microbial assimilation, processing, and fate of MPB-derived N (MPB-N) remains poorly characterised. In this study, an MPB-dominated microbial community was labeled in situ with a pulse of 15NH4+-N. Laboratory core incubations of this labeled sediment under increasing nutrient concentrations (NH4+ and PO43−: ambient, 2 × ambient, 5 × ambient, and 10 × ambient) were used to investigate changes in the processing and flux pathways of the 15N-labeled MPB-N across 10.5 d under nutrient enrichment. Short-term retention of MPB-N by MPB was stimulated by nutrient addition, with higher 15N in MPB in the nutrient amended treatments (71–93%) than in the ambient treatment (38%) at 0.5 d After 10.5 d, the nutrient amended treatments had increased turnover of MPB-N out of MPB biomass into an uncharacterised pool of sediment ON (45–75%). Increased turnover of MPB-N likely resulted from decreased recycling of MPB-N between MPB and heterotrophic bacteria as inorganic nutrients were preferentially used as an N source and remineralisation of sediment ON decreased. Decreased breakdown of sediment ON reduced the efflux of MPB-N via DON in the amended (3.9–5.2%) versus the ambient treatment (10.9%). Exports of MPB-N to the water column were relatively small, accounting for a maximum of 14% of 15N exported from the sediment, and were predominantly exported DON and N2 (denitrification). Overall, there was considerable retention of MPB-N over 10.5 d, but increased nutrient loading shifted N from MPB biomass into other sediment ON.