Roseomonas algicola sp. nov., isolated from a green alga, Pediastrum duplex

A Gram-stain-negative, strictly aerobic bacterium, designated strain PeD5 , was isolated from a green alga from the Nakdong river of the Republic of Korea. Cells were non-motile cocci, catalase-negative and oxidase-positive. Growth of PeD5 was observed at 25-40 °C (optimum, 35 °C) and pH 5.0-10.0 (optimum, pH 7-8), and in the presence of 0-0.25% (w/v) NaCl (optimum, 0%). PeD5 contained C , C ω7 11-methyl, summed feature 3 (comprising C ω7 and/or C ω6 ) and summed feature 8 (comprising C ω7 and/or C ω6 ) as major cellular fatty acids (>5%) and ubiquinone-10 as the sole isoprenoid quinone. Phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an unidentified phospholipid and an unidentified aminolipid were detected as major polar lipids. The genomic DNA G+C content of PeD5 was 71.0 mol%. PeD5 was most closely related to HS-69 with a 97.6% 16S rRNA sequence similarity and shared less than 96.3% 16S rRNA sequence similarities with type strains of other species. Phylogenetic analysis based on 16S rRNA gene sequences indicated that PeD5 formed a phyletic lineage with HS-69 within the genus . On the basis of results of phenotypic, chemotaxonomic and molecular analysis, strain PeD5 clearly represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is PeD5 (=KACC 19925 =JCM 33309 ).