A study to compare Hematopoietic Progenitor Cell count determined on a next‐generation automated cell counter with flow cytometric CD34 count in peripheral blood and the harvested peripheral blood stem cell graft from autologous and allogenic donors

Introduction A successful bone marrow transplant requires a minimum of 2‐4 × 106 cells/kg patient body weight of CD 34+ cells to be transfused, where peripheral blood CD34+ cell count being and ideal predictor. We compared the correlation and predictive capacity of both hematopoietic progenitor cell count (HPC) determined on the Sysmex XN‐9000 and flow cytometric CD34 in autologous and allogenic donors. Methods Autologous and allogenic donors were taken as per criteria. TLC (Total Leukocyte Count), MNC (Mononuclear cell count), HPC, and CD34 assay were done in both the peripheral blood prior to apheresis, and the harvest product postapheresis. Sysmex XN‐9000 was used for TLC, MNC, and HPC tests, and a modified ISH‐AGE protocol was used to enumerate CD34 by flow cytometry. Statistical analysis was done using SPSS 16.0. Results Sixty‐seven allogenic and 35 autologous donors were enrolled. 45% were females, and 55% were males. Correlation between HPC and CD34 was found to be 0.887 with P value