Novel probiotic-bound oxidized Bletilla striata polysaccharide-chitosan composite hydrogel

As a new strategy for wound management, probiotics are highly sensitive to the external environment during use. In this study, an attempt is made to apply oxidized Bletilla striata polysaccharide (OBSP) hydrogel as a delivery system to put up a physical barrier to probiotics. Bletilla striata, as a famous traditional Chinese medicine, has been widely used for over 1500 years to promote wound healing. Firstly, probiotic-bound OBSP-Chitosan composite hydrogel (OBSP-CS-LP hydrogel) was prepared by a simple and environmentally friendly approach. Subsequently, the SEM image, swelling and rheological properties of the OBSP-CS-LP hydrogel were investigated. Notably, composite probiotics to wounds are an attractive novel intervention that significantly improves the antibacterial properties of the hydrogel and avoids the pitfalls of many antibiotic therapies. Furthermore, the full-thickness skin defect model in vivo indicated an outstanding wound healing efficacy. Therefore, OBSP-CS-LP hydrogel could be applied as a promising dressing in the wound healing. [Display omitted] •Novel hydrogel was prepared by a simple and environmentally friendly approach.•The probiotic-based therapies without using commonly antimicrobials have been highlighted in wound treatment.•The innovative composite probiotics in hydrogels for antibacterial shown excellent results.•Composite Lactobacillus plantarum created instructive slightly acid environment for wound healing.