UV-Vis detection of hydrogen peroxide using horseradish peroxidase/copper phosphate hybrid nanoflowers

A colorimetric platform based on enzyme-inorganic hybrid nanoflowers was used for UV–vis colorimetric detection of hydrogen peroxide. [Display omitted] •HRP/Cu3(PO4)2 hybrid nanoflowers were fabricated via self-assembly.•The hybrid nanoflowers showed wide H2O2 detection range from100 nM to 100 μM.•The hybrid nanoflowers exhibited good selectivity for H2O2 detection. A colorimetric platform based on enzyme-inorganic hybrid nanoflowers was used for ultraviolet-visible detection of hydrogen peroxide (H2O2). The hybrid nanoflowers were prepared via incubation at room temperature using horseradish peroxidase (HRP) and copper phosphate (Cu3(PO4)2) as the organic and inorganic components, respectively. HRP/Cu3(PO4)2 hybrid nanoflowers (HRP-HNFs) showed linear H2O2 detection range from 100 nM to 100 μM, moreover, HRP-HNFs exhibited good selectivity for H2O2 detection due to the specificity of HRP. In addition, HRP-HNFs have good reusability, excellent color stability and long-term storage stability, which indicate HRP-HNFs are promising for catalysis and biomedical applications.