Influence of probiotics on biofilm formation and diversity of bacteria colonising crop sorghum ensiled with unsalable vegetables

The objective of this study was to characterise in situ digestion kinetics and bacterial colonisation of crop sorghum ensiled with unsalable carrot or pumpkin at 0, 20 or 40% dry matter (DM). Silages with or without the application of a commercial probiotic were incubated in situ for 0, 3, 6, 9, 24...

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Veröffentlicht in:	Applied microbiology and biotechnology 2020-10, Vol.104 (20), p.8825-8836
Hauptverfasser:	Forwood, Daniel L., Bryce, Elizabeth K., Caro, Eleonora, Holman, Devin B., Meale, Sarah J., Chaves, Alex V.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacteria Bacteria - genetics Biofilms Biomedical and Life Sciences Biotechnology Digestion Fermentation Gastrointestinal agents Genomics Life Sciences Microbial Genetics and Genomics Microbiology Probiotics Proteomics Refuse Disposal RNA RNA, Ribosomal, 16S - genetics RNA, Ribosomal, 16S - metabolism Rumen Rumen - metabolism Silage Sorghum Transcriptomics Vegetables Zea mays
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Beschreibung
Zusammenfassung:	The objective of this study was to characterise in situ digestion kinetics and bacterial colonisation of crop sorghum ensiled with unsalable carrot or pumpkin at 0, 20 or 40% dry matter (DM). Silages with or without the application of a commercial probiotic were incubated in situ for 0, 3, 6, 9, 24 and 48 h. Calculation of in situ digestion kinetics was conducted for DM, organic matter and neutral detergent fibre (aNDF). The V4 region of the 16S rRNA gene was sequenced to determine the composition and diversity of bacteria colonising the silage. Organic matter and DM digestion kinetics indicated that greater vegetable inclusion increased ( P
ISSN:	0175-7598 1432-0614
DOI:	10.1007/s00253-020-10877-5