Highly porous terpolymer-ZIF8@BA MOF composite for identification of mono- and multi-glycosylated peptides/proteins using MS-based bottom-up approach
A hydrophilic terpolymer MOF composite is designed with high surface area and porosity to enrich mono- and multi-glycosylated peptides facilitating a bottom-up approach. Terpolymer@ZIF-8 is synthesized using free radical polymerization followed by layer by layer ZIF-8 fabrication. Subsequent surface...
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Veröffentlicht in: | Mikrochimica acta (1966) 2020-10, Vol.187 (10), p.555-555, Article 555 |
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container_title | Mikrochimica acta (1966) |
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creator | Saleem, Shafaq Sajid, Muhammad Salman Hussain, Dilshad Fatima, Batool Jabeen, Fahmida Najam-ul-Haq, Muhammad Saeed, Adeela |
description | A hydrophilic terpolymer MOF composite is designed with high surface area and porosity to enrich mono- and multi-glycosylated peptides facilitating a bottom-up approach. Terpolymer@ZIF-8 is synthesized using free radical polymerization followed by layer by layer ZIF-8 fabrication. Subsequent surface modification was made by aminophenylboronic acid (AMBA). The enrichment ability of terpolymer@ZIF-8@BA is evaluated by using tryptic digest of IgG and HRP to exemplify mono- and multi-glycosylated protein samples. Improved selectivity of 1:200 for spiked HRP in BSA digest and sensitivity down to 1 fmol μL
−1
is achieved. Batch to batch reproducibility is better 1% RSD which favors the adoption of the developed method for routine N-linked glycopeptide/protein determination. Cost-effective nature of given approach is given by regeneration of the material up to four cycles. Total 318 N-linked glycopeptides have been identified from 1 μL human serum digest after subjecting the enriched and PNGase-treated deglycosylated peptides to LC-MS. Thus, terpolymer@ZIF-8@BA holds the potential both for mono- and multi-glycosylated peptides from complex biological sample.
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doi_str_mv | 10.1007/s00604-020-04532-z |
format | Article |
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−1
is achieved. Batch to batch reproducibility is better 1% RSD which favors the adoption of the developed method for routine N-linked glycopeptide/protein determination. Cost-effective nature of given approach is given by regeneration of the material up to four cycles. Total 318 N-linked glycopeptides have been identified from 1 μL human serum digest after subjecting the enriched and PNGase-treated deglycosylated peptides to LC-MS. Thus, terpolymer@ZIF-8@BA holds the potential both for mono- and multi-glycosylated peptides from complex biological sample.
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−1
is achieved. Batch to batch reproducibility is better 1% RSD which favors the adoption of the developed method for routine N-linked glycopeptide/protein determination. Cost-effective nature of given approach is given by regeneration of the material up to four cycles. Total 318 N-linked glycopeptides have been identified from 1 μL human serum digest after subjecting the enriched and PNGase-treated deglycosylated peptides to LC-MS. Thus, terpolymer@ZIF-8@BA holds the potential both for mono- and multi-glycosylated peptides from complex biological sample.
Graphical abstract</description><subject>Analytical Chemistry</subject><subject>Antibiotics</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Enrichment</subject><subject>Free radical polymerization</subject><subject>Free radicals</subject><subject>Glycopeptides</subject><subject>Microengineering</subject><subject>Nanochemistry</subject><subject>Nanotechnology</subject><subject>Original Paper</subject><subject>Peptides</subject><subject>Porosity</subject><subject>Proteins</subject><subject>Regeneration</subject><subject>Selectivity</subject><subject>Terpolymers</subject><issn>0026-3672</issn><issn>1436-5073</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kb1uFTEQRi0EEpfAC1BZoqEx8d_a3o4QcUmkRCmAhsbyrr03jnZtY3uLzXvwvji5SJFSUE1zzjej-QB4T_AngrE8LRgLzBGmGGHeMYruX4Ad4UygDkv2EuwwpgIxIelr8KaUO4yJFJTvwJ8Lf7idN5hijmuB1eUU521xGf263KvPX87g9c0ejnFJsfjq4BQz9NaF6ic_mupjgHGCSwwRQRMsXNa5enSYtzGWbTbVWZhcqk0ppynH6nwocC0-HOD1dzSY0oAh1hoXtCZoUmPMePsWvJrMXNy7f_ME_Nx__XF-ga5uvl2en12hkXW0Iq76gVOuKDZMEcJ61zFMhB0s660aiBK0Fz2zpGNiIo5YaSiTSomB21Eqwk7Ax2NuW_t7daXqxZfRzbMJrr1DU86JIIRL2tAPz9C7uObQrnukOolp_xBIj9SYYynZTTplv5i8aYL1Q1P62JRuTenHpvR9k9hRKg0OB5efov9j_QXds5di</recordid><startdate>20201001</startdate><enddate>20201001</enddate><creator>Saleem, Shafaq</creator><creator>Sajid, Muhammad Salman</creator><creator>Hussain, Dilshad</creator><creator>Fatima, Batool</creator><creator>Jabeen, Fahmida</creator><creator>Najam-ul-Haq, Muhammad</creator><creator>Saeed, Adeela</creator><general>Springer Vienna</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>20201001</creationdate><title>Highly porous terpolymer-ZIF8@BA MOF composite for identification of mono- and multi-glycosylated peptides/proteins using MS-based bottom-up approach</title><author>Saleem, Shafaq ; Sajid, Muhammad Salman ; Hussain, Dilshad ; Fatima, Batool ; Jabeen, Fahmida ; Najam-ul-Haq, Muhammad ; Saeed, Adeela</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c352t-489b424820a381139e53016dbd39d8b18629693d1536f1e1d7a237886b4dc7813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Analytical Chemistry</topic><topic>Antibiotics</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Enrichment</topic><topic>Free radical polymerization</topic><topic>Free radicals</topic><topic>Glycopeptides</topic><topic>Microengineering</topic><topic>Nanochemistry</topic><topic>Nanotechnology</topic><topic>Original Paper</topic><topic>Peptides</topic><topic>Porosity</topic><topic>Proteins</topic><topic>Regeneration</topic><topic>Selectivity</topic><topic>Terpolymers</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saleem, Shafaq</creatorcontrib><creatorcontrib>Sajid, Muhammad Salman</creatorcontrib><creatorcontrib>Hussain, Dilshad</creatorcontrib><creatorcontrib>Fatima, Batool</creatorcontrib><creatorcontrib>Jabeen, Fahmida</creatorcontrib><creatorcontrib>Najam-ul-Haq, Muhammad</creatorcontrib><creatorcontrib>Saeed, Adeela</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Mikrochimica acta (1966)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saleem, Shafaq</au><au>Sajid, Muhammad Salman</au><au>Hussain, Dilshad</au><au>Fatima, Batool</au><au>Jabeen, Fahmida</au><au>Najam-ul-Haq, Muhammad</au><au>Saeed, Adeela</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Highly porous terpolymer-ZIF8@BA MOF composite for identification of mono- and multi-glycosylated peptides/proteins using MS-based bottom-up approach</atitle><jtitle>Mikrochimica acta (1966)</jtitle><stitle>Microchim Acta</stitle><date>2020-10-01</date><risdate>2020</risdate><volume>187</volume><issue>10</issue><spage>555</spage><epage>555</epage><pages>555-555</pages><artnum>555</artnum><issn>0026-3672</issn><eissn>1436-5073</eissn><abstract>A hydrophilic terpolymer MOF composite is designed with high surface area and porosity to enrich mono- and multi-glycosylated peptides facilitating a bottom-up approach. Terpolymer@ZIF-8 is synthesized using free radical polymerization followed by layer by layer ZIF-8 fabrication. Subsequent surface modification was made by aminophenylboronic acid (AMBA). The enrichment ability of terpolymer@ZIF-8@BA is evaluated by using tryptic digest of IgG and HRP to exemplify mono- and multi-glycosylated protein samples. Improved selectivity of 1:200 for spiked HRP in BSA digest and sensitivity down to 1 fmol μL
−1
is achieved. Batch to batch reproducibility is better 1% RSD which favors the adoption of the developed method for routine N-linked glycopeptide/protein determination. Cost-effective nature of given approach is given by regeneration of the material up to four cycles. Total 318 N-linked glycopeptides have been identified from 1 μL human serum digest after subjecting the enriched and PNGase-treated deglycosylated peptides to LC-MS. Thus, terpolymer@ZIF-8@BA holds the potential both for mono- and multi-glycosylated peptides from complex biological sample.
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subjects | Analytical Chemistry Antibiotics Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Enrichment Free radical polymerization Free radicals Glycopeptides Microengineering Nanochemistry Nanotechnology Original Paper Peptides Porosity Proteins Regeneration Selectivity Terpolymers |
title | Highly porous terpolymer-ZIF8@BA MOF composite for identification of mono- and multi-glycosylated peptides/proteins using MS-based bottom-up approach |
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