Galangin ameliorated pulmonary fibrosis in vivo and in vitro by regulating epithelial-mesenchymal transition

[Display omitted] •Galangin reduced the retention of inflammatory cells.•Galangin inhibited EMT and fibroblast differentiation in mice.•Galangin suppressed TGF-β1-induced fibroblast differentiation in vitro.•Galangin suppressed TGF-β1-induced morphological changes and EMT in A549 cells.•Dietary plan...

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Veröffentlicht in:Bioorganic & medicinal chemistry 2020-10, Vol.28 (19), p.115663-115663, Article 115663
Hauptverfasser: Wang, Liqun, Liu, Hongyao, He, Qiurong, Gan, Cailing, Li, Yali, Zhang, Qianyu, Yao, Yuqin, He, Fang, Ye, Tinghong, Yin, Wenya
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Sprache:eng
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Zusammenfassung:[Display omitted] •Galangin reduced the retention of inflammatory cells.•Galangin inhibited EMT and fibroblast differentiation in mice.•Galangin suppressed TGF-β1-induced fibroblast differentiation in vitro.•Galangin suppressed TGF-β1-induced morphological changes and EMT in A549 cells.•Dietary plant-derived galangin has potential to prevent human lung fibrosis. Pulmonary fibrosis (PF) is a disease that is characterized by abnormal epithelial-mesenchymal transition (EMT) and persistent inflammatory injury, with high mortality and poor prognosis, but the current therapies are accompanied by certain adverse side effects. In this study, we investigated the role of galangin (GA), an anti-inflammatory and anti-tumoral phytochemical extracted from galangal, in preventing and curing bleomycin (BLM)-induced pulmonary fibrosis and the underlying mechanism. Histopathological staining confirmed that GA dramatically moderated bleomycin-induced pulmonary fibrosis in mice. Compared with the vehicle treatment, GA treatment inhibited the expression of vimentin and increased the expression of E-cadherin. The expression of α-Smooth muscle actin (α-SMA), which is a myofibroblast marker, was also suppressed. In addition, GA diminished the increase in the numbers of CD4+CD69+ and CD8+CD69+ T cells and dendritic cells induced by bleomycin, and reduced the residence of inflammatory cells in the lung tissues. Notably, GA inhibited the TGF-β1-induced EMT and fibroblast differentiation in vitro, which further confirmed the potential protective effect of GA on pulmonary fibrosis. Taken together, our results suggest that GA exerts a beneficial effect on bleomycin-induced pulmonary fibrosis by attenuating EMT and inflammatory damage and may have prevent potential of pulmonary fibrosis.
ISSN:0968-0896
1464-3391
DOI:10.1016/j.bmc.2020.115663