A test potential booster for fast-scan cyclic voltammetry with an electrophysiological amplifier

Fast-scan cyclic voltammetry (FSCV) is a powerful technique for studying the local dynamics of neurotransmitters and neuromodulators. FSCV is attractive to researchers employing electrophysiological techniques because it can be performed using an electrophysiological voltage-clamp amplifier. However, the narrow test potential range of electrophysiological amplifiers (typically, ±1 V) limits testable species of analytes. Here we devised a booster that extends the test potential range. Using the booster, we could detect the oxidation current of adenosine peaking near a test potential of +1.5 V. The booster should promote combined electrophysiological and electrochemical studies of synaptic release and neural secretion. [Display omitted] •Fast-scan cyclic voltammetry is useful for studying neurochemical dynamics.•This measurement can be performed using an electrophysiological amplifier.•However, the amplifier's test potential range is too narrow to detect some analytes.•We devised a booster to extend the test potential range.•The booster allows electrophysiologists to analyze a variety of analytes.