Constant light exposure causes oocyte meiotic defects and quality deterioration in mice

Artificial light at night (ALAN) exposes us to prolonged illumination, that adversely affects female reproduction. However, it remains to be clarified how prolonged light exposure affects oocyte meiotic maturation and quality. To this end, we exposed female mice to a constant light (CL) of 250 lux for different durations. Our findings showed that CL exposure for 7 weeks reduced the oocyte maturation rate. Meanwhile, CL exposure caused greater abnormalities in spindle assembly and chromosome alignment and a higher rate of oocyte aneuploidy than the regular light dark cycle. CL exposure also induced oxidative stress and caused mitochondrial dysfunction, which resulted in oocyte apoptosis and autophagy. Notably, our results showed that CL exposure reduced the levels of α-tubulin acetylation, DNA methylation at 5 mC, RNA methylation at m6A and histone methylation at H3K4me2 but increased the levels of histone methylation at H3K27me2 in oocytes. In summary, our findings demonstrate that constant bright light exposure causes oocyte meiotic defects and reduces cytoplasmic quality. These results extend the current understanding of ALAN-mediated defects in female reproduction. [Display omitted] •Constant light exposure causes oocyte meiotic defects and quality deterioration in mice.•Constant light exposure disrupts meiotic organelles, then leads to aneuploidy.•Constant light exposure induces oxidative stress and disturbs epigenetic patterns in oocytes. Constant light exposure compromises oocyte meiotic maturation and quality in female mice, and is associated with defective epigenetic modifications of tubulin, histones and nucleic acids.