Short RNA Universal Coding for Topological Transformation Nano‐barcoding Application

With the advent of innovative genomic discovery toolkits such as RT‐PCR, genetic information can be quickly decrypted, and this has resulted in significant progress in overcoming diseases. However, RT‐PCR has the serious problem of frequent errors, and the demand for a new gene diagnostic system is emerging. Herein, we propose a universal coding system for the effective detection of short single‐stranded DNA or RNA by using a topological transformation‐based nano‐barcoding technique (TNT). Our goal was to develop a dedicated diagnostic device that unifies the other gene groups, thus resulting in minimum testing. In a universal coding system consisting of two separate circulation structures, different gene groups become generalized into specific single genes with the same sequence by a strand‐displacement reaction and are then amplified, eventually being quickly detected in one TNT system. Simple gene diagnostic systems like this make high‐speed, point‐of‐care diagnostic technologies, and we are very confident that these will provide clinical gene detection in the near future. One for all: Topological transformation nano‐barcoding technology (TNT) associated with a universal coding system was proved to be effective in profiling short RNA fragments including clinical gene mutants. It is possible to diagnose short genetic biomarkers in one go with one TNT system by making a universal code without designing each probe material.