Analysis of reflux as the aetiology of laryngeal dysplasia progression through a matched case‐control study

Objectives Laryngeal dysplasia (LD) is a precancerous lesion of the larynx. In this study, the laryngeal tissue of patients with laryngeal dysplasia was taken as the research object, and the aetiology of reflux was analysed. Method Patients with laryngeal dysplasia after surgery were selected as our subjects. The levels of pepsin, enterokinase and bilirubin in laryngeal tissue samples of the two groups were detected by immunohistochemical method. Results The OR values (95% CI) of pepsin, enterokinase and bilirubin were 0.67 (0.19‐2.36), 0.80 (0.22‐2.98) and 1.33 (0.30‐5.96), respectively, in the univariate analysis. Besides, in the multivariate analysis, the OR values (95% CI) of pepsin, enterokinase and bilirubin were 0.57 (0.14‐2.30), 0.73 (0.18‐2.92) and 1.40 (0.30‐6.53), respectively. Conclusion Larger sample size should be applied to prospective studies on whether reflux is a risk factor for laryngeal cancer.