Monolithic Poly(styrene-co-divinylbenzene) Columns for Supercritical Fluid Chromatography–Mass Spectrometry Analysis of Polypeptide

The organic polymer-based monolithic columns have been evaluated as the separation media for analysis of peptides using supercritical fluid chromatography–mass spectrometry (SFC–MS). We demonstrate for the first time the SFC–MS separation of a mixture of polypeptides carried out using poly­(styrene-...

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Veröffentlicht in:Analytical chemistry (Washington) 2020-09, Vol.92 (17), p.11525-11529
Hauptverfasser: Zajickova, Zuzana, Nováková, Lucie, Svec, Frantisek
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Sprache:eng
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Zusammenfassung:The organic polymer-based monolithic columns have been evaluated as the separation media for analysis of peptides using supercritical fluid chromatography–mass spectrometry (SFC–MS). We demonstrate for the first time the SFC–MS separation of a mixture of polypeptides carried out using poly­(styrene-co-divinylbenzene) monolithic columns and carbon dioxide/methanol mobile phase. A gradient from 2 to 40% methanol modifier containing 0.1% TFA as an acidic additive was applied for the optimized elution and the separation was achieved in less than 3 min. Selected ion monitoring enabled detection of selected masses characteristic of three ionophoric pentadecapeptide antibiotics gramicidin A, B, and C and their two corresponding isoforms. Furthermore, their identity was confirmed through determination of their [M + 2H]2+, [M + 2Na]2+, and [M + H + Na]2+ ions acquired by positive-ion electrospray ionization-mass spectrometry (ESI-MS).
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.0c02874