Facilitated bio-mineralization of N,N-dimethylformamide in anoxic denitrification system: Long-term performance and biological mechanism

•Complete removal of high-strength DMF could be achieved in anoxic system.•Simultaneous DMF biodegradation and denitrification was technically feasible.•Hydrolysis and mineralization of DMF were accelerated at the presence of nitrate.•Key genes (enzymes) involved in DMF anoxic biodegradation were elucidated.•Mechanism dominating DMF biodegradation and denitrification was revealed. Due to highly recalcitrant and toxicological nature of N,N-dimethylformamide (DMF), efficient removal of DMF is challenging for biological wastewater treatment. In this study, an anoxic denitrification system was developed and continuously operated for 220 days in order to verify the enhanced DMF biodegradation mechanism. As high as 41.05 mM DMF could be thoroughly removed in the anoxic denitrification reactor at hydraulic residence time (HRT) of 24 h, while the total organic carbon (TOC) and nitrate removal efficiencies were as high as 95.7 ± 2.5% and 98.4 ± 1.1%, respectively. Microbial community analyses indicated that the species related to DMF hydrolysis (Paracoccus, Brevundimonas and Chryseobacterium) and denitrification (Paracoccus, Arenimonas, Hyphomicrobium, Aquamicrobium and Bosea) were effectively enriched in the anoxic denitrification system. Transcriptional analysis coupled with enzymatic activity assay indicated that both hydrolysis and mineralization of DMF were largely enhanced in the anoxic denitrification system. Moreover, the occurrence of microbial denitrification distinctly facilitated carbon source utilization to produce electron and energy, which was rather beneficial for better reactor performance. This study demonstrated that the anoxic denitrification system would be a potential alternative for efficient treatment of wastewater polluted by recalcitrant pollutants such as DMF. [Display omitted]