Implantation associated changes in expression profile of indoleamine-2, 3-dioxygenase 1, Th1-Th2 cytokines and interferon-stimulated genes on neutrophils and peripheral blood mononuclear cells of crossbred cows
[Display omitted] •IDO1 and ISGs expression increased in neutrophils and PBMCs of pregnant cows under systemic IFNτ stimulation.•IDO1 modulates the cytokine balance towards anti-inflammatory side and thus facilitates embryo survival.•Controlled IFNγ and TNFα expression might be necessary for increas...
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Veröffentlicht in: | Journal of reproductive immunology 2020-11, Vol.142, p.103188-103188, Article 103188 |
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Sprache: | eng |
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•IDO1 and ISGs expression increased in neutrophils and PBMCs of pregnant cows under systemic IFNτ stimulation.•IDO1 modulates the cytokine balance towards anti-inflammatory side and thus facilitates embryo survival.•Controlled IFNγ and TNFα expression might be necessary for increased IDO1 expression and CL maintenance for successful establishment of pregnancy.
Effective bidirectional communication between the embryo and dam improves the reproductive efficiency of dairy cows. Possible role of immunosuppressive indolamine-2, 3-dioxygenase 1 (IDO1) enzyme in the regulation of maternal systemic cytokine balance/shift during early pregnancy establishment along with various interferon-stimulated genes (ISGs) expression in neutrophils and peripheral blood mononuclear cell (PBMCs) were investigated in crossbred cows. Blood was collected on days 0 i.e. day of Artificial Insemination (AI), 10, 18 and 36 post-AI followed by isolation of neutrophils and PBMCs for gene expression study of IDO1, anti-inflammatory cytokines (IL-4, IL-10 and TGFβ1), pro-inflammatory cytokines (IFNγ and TNFα) and ISGs (ISG15, MX1, MX2, OAS1) in pregnant and non-pregnant cows. Cows were grouped as pregnant and non-pregnant after pregnancy confirmation by non-return to heat, ultrasonography, per rectal examination along with progesterone and IFNτ assay. Significantly (P |
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ISSN: | 0165-0378 1872-7603 |
DOI: | 10.1016/j.jri.2020.103188 |