Physico-mechanical and finite element analysis evaluation of 3D printable alginate-methylcellulose inks for wound healing applications

•Alginate/methylcellulose hydrogel inks with bioactives showed good printability.•A finite element model confirmed the accuracy of the mechanical measurements.•Combined experimental-numerical approach useful in determining mechanical behavior.•The blend hydrogels showed good biocompatibility on human dermal fibroblasts.•In vitro wound healing assay confirmed hydrogels’ potency to stimulate cell growth and migration. The present study reports on the comprehensive physico-mechanical evaluation of 3D printable alginate-methylcellulose hydrogels with bioactive components (Manuka honey, aloe vera gel, eucalyptus essential oil) using a combined experimental-numerical approach. The 3D printable carbohydrate inks demonstrated good swelling properties under moist conditions and adequate antimicrobial and antibiofilm efficacy against both Gram positive and negative bacteria. The effect of the bioactive compounds on the viscosity and mechanical properties of the 3D printable hydrogels was assessed with rheological, nanoindentation and shear test measurements. All hydrogel compositions showed good biocompatibility on human dermal fibroblasts, stimulating cell growth as confirmed by an in vitro wound healing assay. Finite element analysis simulation was employed to further advance the calculation accuracy of the nanoindentation tests, concluding that combination of an experimental and a numerical technique may constitute a useful method to characterize the mechanical behavior of composite hydrogel films for use in wound healing applications.