Screening of toll-like receptor signaling pathway-related genes and the response of recombinant glutathione S-transferase A3 protein to thiram induced apoptosis in chicken erythrocytes

The expression of genes related to the Toll-like receptors (TLRs) signaling pathway were determined. Group A, B and C fed with basal diet and group D, E and F induced TD by feeding a basal diet containing 100 mg·kg−1 thiram. rGSTA3 protein was injected at 20 μg·kg−1 in group B, E and at 50 μg·kg−1 i...

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Veröffentlicht in:	Developmental and comparative immunology 2021-01, Vol.114, p.103831-103831, Article 103831
Hauptverfasser:	Jahejo, Ali Raza, Jia, Fa-jie, Raza, Sayed Haidar Abbas, Shah, Mujahid Ali, Yin, Jiao-jiao, Ahsan, Anam, Waqas, Muhammad, Niu, Sheng, Ning, Guan-bao, Zhang, Ding, Khan, Ajab, Tian, Wen-xia
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Apoptosis Avian Proteins - genetics Avian Proteins - metabolism Broiler Chickens - immunology Chondrocytes Chondrocytes - physiology Diet Erythrocyte Erythrocytes Erythrocytes - physiology Gene expression Genes Glutathione Glutathione transferase Glutathione Transferase - genetics Glutathione Transferase - metabolism Immunity Immunity, Innate Interleukin 7 MyD88 protein Osteochondrodysplasias - immunology Poultry Diseases - immunology Protein Proteins Receptors Recombinant Proteins - metabolism Signal transduction Signal Transduction - genetics Signaling Thiram Thiram - metabolism Tibial dyschondroplasia TLR2 protein TLR3 protein TLR5 protein TLR7 protein Toll-like receptors Toll-Like Receptors - metabolism TRAF6 protein Transcriptome
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Zusammenfassung:	The expression of genes related to the Toll-like receptors (TLRs) signaling pathway were determined. Group A, B and C fed with basal diet and group D, E and F induced TD by feeding a basal diet containing 100 mg·kg−1 thiram. rGSTA3 protein was injected at 20 μg·kg−1 in group B, E and at 50 μg·kg−1 in C, F. Results suggested that lameness and death of chondrocytes were significant on day 14. TLRs signaling pathway related genes were screened based on the transcriptome enrichment, and validated on qPCR. IL-7, TLR2, 3, 4, 5, 7, 15, MyD88, MHC-II, MDA5 and TRAF6 were significantly (p
ISSN:	0145-305X 1879-0089
DOI:	10.1016/j.dci.2020.103831