A novel circular RNA hsa_circ_0060927 may serve as a potential diagnostic biomarker for human colorectal cancer

A novel circular RNA hsa_circ_0060927 may serve as a potential diagnostic biomarker for human colorectal cancer

CYP24A1 plays a role in strictly regulated vitamin D metabolism pathway and has been nominated as a prognostic biomarker for colorectal cancer (CRC). Increasing evidence suggests that circular RNAs (circRNAs) are involved in cancer initiation and progression; however, their diagnostic values in canc...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Molecular biology reports 2020-09, Vol.47 (9), p.6649-6655
Hauptverfasser: Sadeghi, Hossein, Heiat, Mohammad
Format: Artikel
Sprache:eng
Schlagworte:
Animal Anatomy
Animal Biochemistry
Biomarkers
Biomedical and Life Sciences
Circular RNA
Colon cancer
Colorectal cancer
Colorectal carcinoma
Histology
Life Sciences
Morphology
Original Article
Polymerase chain reaction
Transcription
Tumor cell lines
Vitamin D
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 6655
container_issue 9
container_start_page 6649
container_title Molecular biology reports
container_volume 47
creator Sadeghi, Hossein
Heiat, Mohammad
description CYP24A1 plays a role in strictly regulated vitamin D metabolism pathway and has been nominated as a prognostic biomarker for colorectal cancer (CRC). Increasing evidence suggests that circular RNAs (circRNAs) are involved in cancer initiation and progression; however, their diagnostic values in cancers as the new potential biomarkers are still not fully understood. In the present study, by using quantitative real-time polymerase chain reaction (qRT-PCR), we demonstrated that CYP24A1 and hsa_circ_0060927 were only transcribed in 33 and 25 out of 83 CRC tissues, respectively. In these samples, we demonstrated that CYP24A1 was up-regulated in both linear and circular forms ( P  
doi_str_mv 10.1007/s11033-020-05716-9
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2434748421</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2577745704</sourcerecordid><originalsourceid>FETCH-LOGICAL-c375t-23ffaf37609342a20f01a139107e60b5bcf91e256de915be8f39ed4b09216563</originalsourceid><addsrcrecordid>eNp9kctO3TAQhq2qVTlcXoBFZYlNN6EzvsTJ8gi1BQm1EmJvOT5jCE3ig50g8fYYDrQSi65Gmvnmn8vP2DHCKQKYbxkRpKxAQAXaYF21H9gKtZGVak3zka1AAlaq0bjH9nO-AwCFRn9me1I0IDWIFYtrPsUHGrjvk18Gl_jVrzW_zc4-JyxADa0wfHSPPFN6IO4yd3wbZ5rm3g1807ubKea597zr4-jSH0o8xMRvl9FN3MchJvJzIb2bPKVD9im4IdPRazxg1z--X5-dV5e_f16crS8rL42eKyFDcEGaMl0q4QQEQIeyRTBUQ6c7H1okoesNtag7aoJsaaO6sizWupYH7OtOdpvi_UJ5tmOfPQ2Dmygu2QollVGNEljQk3foXVzSVJazQhtjlDagCiV2lE8x50TBblNfzn20CPbZDbtzwxY37Isbti1NX16ll26kzd-Wt_cXQO6AXErTDaV_s_8j-wTOy5Mg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2577745704</pqid></control><display><type>article</type><title>A novel circular RNA hsa_circ_0060927 may serve as a potential diagnostic biomarker for human colorectal cancer</title><source>SpringerNature Journals</source><creator>Sadeghi, Hossein ; Heiat, Mohammad</creator><creatorcontrib>Sadeghi, Hossein ; Heiat, Mohammad</creatorcontrib><description>CYP24A1 plays a role in strictly regulated vitamin D metabolism pathway and has been nominated as a prognostic biomarker for colorectal cancer (CRC). Increasing evidence suggests that circular RNAs (circRNAs) are involved in cancer initiation and progression; however, their diagnostic values in cancers as the new potential biomarkers are still not fully understood. In the present study, by using quantitative real-time polymerase chain reaction (qRT-PCR), we demonstrated that CYP24A1 and hsa_circ_0060927 were only transcribed in 33 and 25 out of 83 CRC tissues, respectively. In these samples, we demonstrated that CYP24A1 was up-regulated in both linear and circular forms ( P  &lt; 0.05). Furthermore, we showed that CYP24A1 and hsa_circ_0060927 transcription were induced after vitamin D treatment in HCT-116 and HT-29 colon cancer cell lines. Importantly, analysis of the ROC curve in expressed samples indicated that CYP24A1 and hsa_circ_0060927 could serve as the potential diagnostic markers of CRC, but due to the fact that these transcripts are not expressed in many samples, the diagnostic role of these transcripts could be rejected. Taken together, for the first time, the results of our study illustrated the expression levels hsa_circ_0060927 in CRC; however, further studies are required to validate the present results.</description><identifier>ISSN: 0301-4851</identifier><identifier>EISSN: 1573-4978</identifier><identifier>DOI: 10.1007/s11033-020-05716-9</identifier><identifier>PMID: 32803502</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animal Anatomy ; Animal Biochemistry ; Biomarkers ; Biomedical and Life Sciences ; Circular RNA ; Colon cancer ; Colorectal cancer ; Colorectal carcinoma ; Histology ; Life Sciences ; Morphology ; Original Article ; Polymerase chain reaction ; Transcription ; Tumor cell lines ; Vitamin D</subject><ispartof>Molecular biology reports, 2020-09, Vol.47 (9), p.6649-6655</ispartof><rights>Springer Nature B.V. 2020</rights><rights>Springer Nature B.V. 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-23ffaf37609342a20f01a139107e60b5bcf91e256de915be8f39ed4b09216563</citedby><cites>FETCH-LOGICAL-c375t-23ffaf37609342a20f01a139107e60b5bcf91e256de915be8f39ed4b09216563</cites><orcidid>0000-0001-9599-6151</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11033-020-05716-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11033-020-05716-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32803502$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sadeghi, Hossein</creatorcontrib><creatorcontrib>Heiat, Mohammad</creatorcontrib><title>A novel circular RNA hsa_circ_0060927 may serve as a potential diagnostic biomarker for human colorectal cancer</title><title>Molecular biology reports</title><addtitle>Mol Biol Rep</addtitle><addtitle>Mol Biol Rep</addtitle><description>CYP24A1 plays a role in strictly regulated vitamin D metabolism pathway and has been nominated as a prognostic biomarker for colorectal cancer (CRC). Increasing evidence suggests that circular RNAs (circRNAs) are involved in cancer initiation and progression; however, their diagnostic values in cancers as the new potential biomarkers are still not fully understood. In the present study, by using quantitative real-time polymerase chain reaction (qRT-PCR), we demonstrated that CYP24A1 and hsa_circ_0060927 were only transcribed in 33 and 25 out of 83 CRC tissues, respectively. In these samples, we demonstrated that CYP24A1 was up-regulated in both linear and circular forms ( P  &lt; 0.05). Furthermore, we showed that CYP24A1 and hsa_circ_0060927 transcription were induced after vitamin D treatment in HCT-116 and HT-29 colon cancer cell lines. Importantly, analysis of the ROC curve in expressed samples indicated that CYP24A1 and hsa_circ_0060927 could serve as the potential diagnostic markers of CRC, but due to the fact that these transcripts are not expressed in many samples, the diagnostic role of these transcripts could be rejected. Taken together, for the first time, the results of our study illustrated the expression levels hsa_circ_0060927 in CRC; however, further studies are required to validate the present results.</description><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Biomarkers</subject><subject>Biomedical and Life Sciences</subject><subject>Circular RNA</subject><subject>Colon cancer</subject><subject>Colorectal cancer</subject><subject>Colorectal carcinoma</subject><subject>Histology</subject><subject>Life Sciences</subject><subject>Morphology</subject><subject>Original Article</subject><subject>Polymerase chain reaction</subject><subject>Transcription</subject><subject>Tumor cell lines</subject><subject>Vitamin D</subject><issn>0301-4851</issn><issn>1573-4978</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kctO3TAQhq2qVTlcXoBFZYlNN6EzvsTJ8gi1BQm1EmJvOT5jCE3ig50g8fYYDrQSi65Gmvnmn8vP2DHCKQKYbxkRpKxAQAXaYF21H9gKtZGVak3zka1AAlaq0bjH9nO-AwCFRn9me1I0IDWIFYtrPsUHGrjvk18Gl_jVrzW_zc4-JyxADa0wfHSPPFN6IO4yd3wbZ5rm3g1807ubKea597zr4-jSH0o8xMRvl9FN3MchJvJzIb2bPKVD9im4IdPRazxg1z--X5-dV5e_f16crS8rL42eKyFDcEGaMl0q4QQEQIeyRTBUQ6c7H1okoesNtag7aoJsaaO6sizWupYH7OtOdpvi_UJ5tmOfPQ2Dmygu2QollVGNEljQk3foXVzSVJazQhtjlDagCiV2lE8x50TBblNfzn20CPbZDbtzwxY37Isbti1NX16ll26kzd-Wt_cXQO6AXErTDaV_s_8j-wTOy5Mg</recordid><startdate>20200901</startdate><enddate>20200901</enddate><creator>Sadeghi, Hossein</creator><creator>Heiat, Mohammad</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-9599-6151</orcidid></search><sort><creationdate>20200901</creationdate><title>A novel circular RNA hsa_circ_0060927 may serve as a potential diagnostic biomarker for human colorectal cancer</title><author>Sadeghi, Hossein ; Heiat, Mohammad</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-23ffaf37609342a20f01a139107e60b5bcf91e256de915be8f39ed4b09216563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animal Anatomy</topic><topic>Animal Biochemistry</topic><topic>Biomarkers</topic><topic>Biomedical and Life Sciences</topic><topic>Circular RNA</topic><topic>Colon cancer</topic><topic>Colorectal cancer</topic><topic>Colorectal carcinoma</topic><topic>Histology</topic><topic>Life Sciences</topic><topic>Morphology</topic><topic>Original Article</topic><topic>Polymerase chain reaction</topic><topic>Transcription</topic><topic>Tumor cell lines</topic><topic>Vitamin D</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sadeghi, Hossein</creatorcontrib><creatorcontrib>Heiat, Mohammad</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular biology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sadeghi, Hossein</au><au>Heiat, Mohammad</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A novel circular RNA hsa_circ_0060927 may serve as a potential diagnostic biomarker for human colorectal cancer</atitle><jtitle>Molecular biology reports</jtitle><stitle>Mol Biol Rep</stitle><addtitle>Mol Biol Rep</addtitle><date>2020-09-01</date><risdate>2020</risdate><volume>47</volume><issue>9</issue><spage>6649</spage><epage>6655</epage><pages>6649-6655</pages><issn>0301-4851</issn><eissn>1573-4978</eissn><abstract>CYP24A1 plays a role in strictly regulated vitamin D metabolism pathway and has been nominated as a prognostic biomarker for colorectal cancer (CRC). Increasing evidence suggests that circular RNAs (circRNAs) are involved in cancer initiation and progression; however, their diagnostic values in cancers as the new potential biomarkers are still not fully understood. In the present study, by using quantitative real-time polymerase chain reaction (qRT-PCR), we demonstrated that CYP24A1 and hsa_circ_0060927 were only transcribed in 33 and 25 out of 83 CRC tissues, respectively. In these samples, we demonstrated that CYP24A1 was up-regulated in both linear and circular forms ( P  &lt; 0.05). Furthermore, we showed that CYP24A1 and hsa_circ_0060927 transcription were induced after vitamin D treatment in HCT-116 and HT-29 colon cancer cell lines. Importantly, analysis of the ROC curve in expressed samples indicated that CYP24A1 and hsa_circ_0060927 could serve as the potential diagnostic markers of CRC, but due to the fact that these transcripts are not expressed in many samples, the diagnostic role of these transcripts could be rejected. Taken together, for the first time, the results of our study illustrated the expression levels hsa_circ_0060927 in CRC; however, further studies are required to validate the present results.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>32803502</pmid><doi>10.1007/s11033-020-05716-9</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0001-9599-6151</orcidid></addata></record>
fulltext fulltext
identifier ISSN: 0301-4851
ispartof Molecular biology reports, 2020-09, Vol.47 (9), p.6649-6655
issn 0301-4851
1573-4978
language eng
recordid cdi_proquest_miscellaneous_2434748421
source SpringerNature Journals
subjects Animal Anatomy
Animal Biochemistry
Biomarkers
Biomedical and Life Sciences
Circular RNA
Colon cancer
Colorectal cancer
Colorectal carcinoma
Histology
Life Sciences
Morphology
Original Article
Polymerase chain reaction
Transcription
Tumor cell lines
Vitamin D
title A novel circular RNA hsa_circ_0060927 may serve as a potential diagnostic biomarker for human colorectal cancer
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-20T07%3A48%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20novel%20circular%20RNA%20hsa_circ_0060927%20may%20serve%20as%20a%20potential%20diagnostic%20biomarker%20for%20human%20colorectal%20cancer&rft.jtitle=Molecular%20biology%20reports&rft.au=Sadeghi,%20Hossein&rft.date=2020-09-01&rft.volume=47&rft.issue=9&rft.spage=6649&rft.epage=6655&rft.pages=6649-6655&rft.issn=0301-4851&rft.eissn=1573-4978&rft_id=info:doi/10.1007/s11033-020-05716-9&rft_dat=%3Cproquest_cross%3E2577745704%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2577745704&rft_id=info:pmid/32803502&rfr_iscdi=true