Noncanonical Amino Acids in Synthetic Biosafety and Post‐translational Modification Studies

The incorporation of noncanonical amino acids (ncAAs) has been extensively studied because of its broad applicability. In the past decades, various in vitro and in vivo ncAA incorporation approaches have been developed to generate synthetic recombinant proteins. Herein, we discuss the methodologies for ncAA incorporation, and their use in diverse research areas, such as in synthetic biosafety and for studies of post‐translational modifications. Trying something different: Specially designed noncanonical amino acids (ncAAs) are highly useful building blocks. This minireview explores how noncanonical chemical groups can be incorporated into proteins, and how these methodologies may be applied in studies of post‐translational modifications and synthetic biosafety.