Real-Time Intracellular Temperature Imaging Using Lanthanide-Bearing Polymeric Micelles

Measurement of thermogenesis in individual cells is a remarkable challenge due to the complexity of the biochemical environment (such as pH and ionic strength) and to the rapid and yet not well-understood heat transfer mechanisms throughout the cell. Here, we present a unique system for intracellula...

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Veröffentlicht in:Nano letters 2020-09, Vol.20 (9), p.6466-6472
Hauptverfasser: Piñol, Rafael, Zeler, Justyna, Brites, Carlos D. S, Gu, Yuanyu, Téllez, Pedro, Carneiro Neto, Albano N, da Silva, Thiago E, Moreno-Loshuertos, Raquel, Fernandez-Silva, Patrício, Gallego, Ana Isabel, Martinez-Lostao, Luis, Martínez, Abelardo, Carlos, Luís D, Millán, Angel
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Sprache:eng
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Zusammenfassung:Measurement of thermogenesis in individual cells is a remarkable challenge due to the complexity of the biochemical environment (such as pH and ionic strength) and to the rapid and yet not well-understood heat transfer mechanisms throughout the cell. Here, we present a unique system for intracellular temperature mapping in a fluorescence microscope (uncertainty of 0.2 K) using rationally designed luminescent Ln3+-bearing polymeric micellar probes (Ln = Sm, Eu) incubated in breast cancer MDA-MB468 cells. Two-dimensional (2D) thermal images recorded increasing the temperature of the cells culture medium between 296 and 304 K shows inhomogeneous intracellular temperature progressions up to ∼20 degrees and subcellular gradients of ∼5 degrees between the nucleolus and the rest of the cell, illustrating the thermogenic activity of the different organelles and highlighting the potential of this tool to study intracellular processes.
ISSN:1530-6984
1530-6992
DOI:10.1021/acs.nanolett.0c02163