Application of single-cell RNA sequencing in embryonic development

Embryonic development is a complex process that is regulated by a series of precise cellular behaviours. The limited number of cells in the early stages of embryonic development represents a challenge for studying early gene regulation and maintaining cell sternness. Single-cell sequencing is a new technology for high-throughput sequencing analysis at the single-cell level that not only reflects the heterogeneity between cells but also reveals gene expression characteristics in different cells from limited samples. Currently, the widespread application of single-cell RNA sequencing technology is gradually changing our understanding of disease pathogenesis. This article reviews the application of single-cell RNA sequencing in embryonic development in recent years and provides innovative ideas for research on embryonic development and the treatment of diseases related to embryonic development. •Explore cell heterogeneity, as well as discover new cell types and marker genes.•Reveal the differentiation trajectory of various cell types during embryonic development.•Pathogenesis of embryonic development-related diseases.•New ideas for the research of embryonic development.