Expression profiling of microRNAs in the Mycoplasma bovis infected mammary gland tissue in Holstein Friesian cattle

The immune response associated with mastitis caused by Mycoplasma bovis is a very complicated biological process in several type of cells, including immune cells, mammary epithelial cells, and endothelial cells. Thus, revealing of the microRNAs in the Mycoplasma bovis infected mammary gland tissues is particularly important for the immune response mechanism to Mycoplasma bovis. Firstly, 20 mammary gland tissue samples were collected from Holstein Friesian cattle that was located in Erzurum province at 2018 and screened for Mycoplasma bovis. Then, total RNA was isolated from Mycoplasma bovis infected tissues and high-throughput sequencing was performed. After bioinformatics analysis, GO and KEGG analysis of target genes of identified microRNAs were conducted. In this study, a total of 616 microRNAs were found. Our results revealed that 24 of the known microRNAs were expressed differently and 13 of the novel microRNAs were expressed differently in Mycoplasma bovis positive tissues. The target genes of these microRNAs were found to be associated with especially inflammation pathways, including B cell and T cell receptor signaling, Fc gamma R-mediated, phagocytosis/chemokine signaling, and MAPK signaling. In conclusion, this study demonstrated that identified miRNAs may be involved in the signaling pathways during mastitis caused by Mycoplasma bovis. •The microRNA expression profile in mammary gland tissues of holstein with M. bovis was identified.•525 known microRNAs and 92 novel microRNAs were detected.•24 of the known microRNAs were expressed differently in Mycoplasma bovis positive tissues.•13 of the novel microRNAs were expressed differently in Mycoplasma bovis positive tissues.•The most expressed microRNAs in M. bovis positive samples were found to be bta-let-7 family.